Macherey-Nagel Bioanalysis™ NucleoSpin™ 96 Trace, 96-Well Kit

Beschreibung

Lysis is achieved by incubation of samples in a solution containing chaotropic ions in the presence of proteinase K. If subsequent separation of the lysate from the sample material is difficult, the lysate can be separated from the forensic material under vacuum or centrifugation by use of the NucleoSpin™ Trace filter plate (not included in the kits). Appropriate conditions for binding of DNA to the silica membrane in the NucleoSpin™ Trace binding strip/binding plate are created by addition of isopropanol to the lysate. The binding process is reversible and specific to nucleic acids. Contaminants are removed by two washing steps with ethanolic buffer. Pure genomic DNA is finally eluted under low ionic strength conditions in a slightly alkaline elution buffer.Typically yields of 1 to 2 μg genomic DNA can be purified from buccal swabs. The final concentration of eluted DNA is 10 to 20 ng/μL (depending on elution buffer volume). Typically, A_260/280 is 1.8 to 1.9. The obtained DNA can be used directly as template for PCR.

Kit components

NucleoSpin™ Trace binding strips, MN wash plates, MN square well blocks, rack with tube strips, cap strips, self-adhering foil, buffers, proteinase K. NucleoSpin™ 96 Trace - NucleoSpin™ Trace binding plates, MN wash plates, MN square well blocks, racks with tube strips, cap strips, buffers, proteinase K.

• Suitable for manual and automated processing, processing possible under vacuum or by centrifugation
• 96 well plate format
• Typical yield: 1 to 2 μg DNA from buccal swabs
• Elution volume: 50 to 100 μL
• Silica membrane technology
• No use of organic solvents
• Highly pure nucleic acids suitable for all common downstream applications
• Preparation time: 70 min/plate
• Binding capacity: 20 μg