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Ly-51 Rat anti-Mouse, FITC, Clone: 6C3, BD
Rat Monoclonal Antibody
Marke: BD Biosciences 562057
Additional Details : Gewicht : 0.01000kg
Beschreibung
The 6C3 antibody reacts with an epitope of the 6C3/BP-1 (Ly-51) glycoprotein cell-surface differentiation antigen, which was originally identified on pre-B cell lymphomas (spontaneous and chemical- or retrovirus- transformed, in vitro and in vivo). 6C3/BP-1 is a homodimer cell-surface glycoprotein with 140-kDa subunits which has been identified to possess aminopeptidase A (APA) activity. The same antigen is expressed at high levels on bone marrow stromal cell lines which support in vitro B lymphopoieses, on thymic dendritic cells and cortical epithelial cells, and on a wide variety of mouse and rat tissues known to possess APA activity. Subsets of normal bone marrow pre-B and B lymphocytes express low levels of Ly-51, which is rapidly up-regulated on the pre-B cells in the presence of IL-7. A role for the 6C3/BP-1 molecule in the IL-7-driven proliferation of B cell precursors has been postulated. However, B-cell abnormalities were not detected in Ly-51-deficient mice. Mature B lymphocytes, thymocytes, peripheral T lymphocytes, erythroid cells, and myeloid cells (with the exception of thymic dendritic cells) do not express Ly-51. The 6C3 antibody can be used to identify cortical epithelium in frozen sections of thymuses from normal, SCID, and TCR-transgenic mice. It is possible that the low level of 6C3/BP-1 antigen detected, by flow cytometry, on some thymocytes may be passively adsorbed from adjacent epithelial cells during preparation of the cell suspensions.
Spezifikation
Ly-51 | |
Monoclonal | |
0.5mg/mL | |
6C3/BP-1 Antigen | |
Rat | |
Affinity Purified | |
RUO | |
Mouse | |
IgG2a κ |
Flow Cytometry | |
6C3 | |
FITC | |
Aqueous buffered solution containing ≤0.09% sodium azide. | |
C57L mouse Pre-B lymphhoma cell line L1-2 | |
50 μg | |
Primary | |
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
For Research Use Only.