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Novus Biologicals™ FoxP3
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Menge:
2μ g
Packungsgröße:
2 Mikrogramm
Beschreibung
- RetroMax system takes advantages of two properties of 293 cells (high level of transfectibility and strong E1A-mediated stimulation of CMV promoter controlled transcription)
- 293 cells of nonmurine origin avoid problem of selective packaging and transfer of VL30 genomes (present in all murine packaging cells)
- Vector supernatants are free of helper virus and are of sufficiently high titer within 2 days of transient transfection in 293 cells
- For amplification, transform DH-5alpha or XL-1 Blue and plate on LB plates containing 50mg/mL ampicillin
- Buffer: 20μL 1x TE (10mM Tris, pH 7.5, 1mM EDTA)
Recommended Storage
Store at -20°C. Avoid freeze-thaw cycles.
Spezifikation
Spezifikation
| Lagerungspuffer | 20µL 1x TE (10mM Tris, pH 7.5, 1mM EDTA) |
| Inhalt und Lagerung | Store at -20°C. Avoid freeze-thaw cycles. |
| Menge | 2μ g |
| Vektor | FoxP3 Vector |
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