Enzyme und Inhibitoren

Thermo Scientific™ T4 DNA-Ligase

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. T4 DNA LIGASE 5U/UL 5X1000U-20°C, 5x1000 units (5u/µl)

Thermo Scientific™ FastDigest BsrDI (BseMI)

Cut at GCAATG(2/0)^ sites with Thermo Scientific™ FastDigest™ BsrDI (BseMI), which performs best with best using universal FastDigest Buffer (Isoschizomers: Bse3DI). FASTDIGEST BSRDI (BSEMI) 20UL

Thermo Scientific™ FastDigest BlpI (Bpu1102I)

Cut at GC^TNAGC sites with Thermo Scientific™ FastDigest™ BlpI (Bpu1102I), which performs best using universal FastDigest Buffer (Isoschizomers: Bsp1720I, CelII). FASTDIGEST BLPI(BPU1102I) 50UL

Lipase AY30, ACROS Organics

CAS: 9001-62-1 25GR Lipase AY30

Invitrogen™ TURBO DNA-free Kit

Ideal tool for clearing residual DNA Ambion® TURBO DNA-free, 50 reactions

Thermo Scientific™ FastDigest™ EcoRI

The FastDigest EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer. X5 FASTDIGEST ECORI 500UL

Thermo Scientific™ FastDigest™ NotI

The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI). 150RXN Thermo Scientific FastDigest NotI restriction enzyme recognizes GC¬GGCCGC site

Invitrogen™ T4 DNA Ligase (5U/uL)

Catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3' hydroxyl and 5' phosphate termini T4 DNA Ligase, 5 units/µl, 250 units

Thermo Scientific™ FastDigest EcoO109I

Cut at RG^GNCCY sites with Thermo Scientific™ FastDigest™ EcoO109I, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: DraII). FASTDIGEST ECOO109I 200UL

Thermo Scientific™ FastDigest AvaII (Eco47I)

Cut at G^GWCC sites with Thermo Scientific™ FastDigest™ AvaII (Eco47I), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer. FASTDIGEST AVAII(ECO47I) 200UL

Thermo Scientific™ Agarase

Specifically digests the agarose polysaccharide core, which allows for gentle yet efficient recovery of DNA or RNA fragments from low melting point agarose. AGARASE 0.5U/UL 100U

Thermo Scientific™ Rinderserumalbumin (BSA)

Stabilize enzymes, increase PCR yields, and prevent adhesion of enzymes with Thermo Scientific™ Bovine Serum Albumin. BSA 20MG/ML 5MG

Thermo Scientific™ CpoI (RsrII)

The CpoI (RsrII) restriction enzyme recognizes CG^GWCCG sites and cuts best at 37°C in Tango buffer (Isoschizomers: CspI, Rsr2I, RsrII). CPOI (RSRII) 10U/UL 1000U

Thermo Scientific™ Pyrophosphatase, anorganisch (aus Hefe)

Catalyze the hydrolysis of inorganic pyrophosphate to two orthophosphates. PYROPHOSPH INORG 10U 0.1U/UL

Thermo Scientific™ RNase A, DNase- und proteasefrei

Specifically degrade single-stranded RNA at C and U residues with the endoribonuclease RNase A. RNASE A DNASE FRE 10MG/ML 10MG

Invitrogen™ DNase I

Purified from bovine pancreas DNase I, 20000 units,(Deoxyribonuclease I)(non-RNA prep.to remove contaminating DNA)

Thermo Scientific™ FastDigest™ HindIII

The FastDigest HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer. X5 FASTDIGEST HINDIII 500UL

Thermo Scientific™ FastDigest™ SacI

The FastDigest SacI restriction enzyme recognizes GAGCT^C sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI) FASTDIGEST SACI 200UL

Thermo Scientific™ Mph1103I (NsiI)

The Mph1103I (NsiI) restriction enzyme recognizes ATGCA^T sites and cuts best at 37°C in R buffer (Isoschizomers: EcoT22I, NsiI, Zsp2I). MPH1103I (NSII) 10U/UL 5000U

OPTIZYME™ SP6 RNA Polymerase, Fisher BioReagents™

2000UNIT Optizyme SP6 RNA Polymerase

Invitrogen™ DNA-free™ DNA Removal Kit

Safely eliminate DNA contamination from RNA samples DNA-free DNase Treatment and Removal Reagent, 50rxns

Thermo Scientific™ FastDigest SbfI (SdaI)

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. FASTDIGEST SBFI (SDAI) 20UL

Thermo Scientific™ ATP

Optimize many molecular biology applications using this ATP, a highly stable nucleotide with 99% purity. ATP 100MM 0.25ML

Thermo Scientific™ Eco47III (AfeI)

The Eco47III (AfeI) restriction enzyme recognizes AGC^GCT sites and cuts best at 37°C in O buffer (Isoschizomers: AfeI, Aor51HI). ECO47III (AFEI) 10U/UL 200U

Thermo Scientific™ FastDigest Bsh1236I

Cut at CG^CG sites with Thermo Scientific™ FastDigest™ Bsh1236I, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer. FASTDIGEST BSH1236I 100UL

Thermo Scientific™ Klenow-Fragment

Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'→3' polymerase, 3'→5' exonuclease (proofreading) activities, but lacking 5'→3' exonuclease activity. KLENOW FR 10U/UL 1500U

Thermo Scientific™ Nb.Mva1269I

Nick at GAATGC sites with Nb.Mva1269I nicking enzyme, which performs best at 37°C in O buffer. NB.MVA1269I 10U/UL 1000U

Thermo Scientific™ RiboLock RNase-Inhibitor

RiboLock RNase Inhibitor specifically inhibits the activity of RNases A, B, and C, protecting RNA under a variety of reaction conditions. RIBOLOCK RI 40U/UL 2500U

Thermo Scientific™ 50x TAE-Puffer (Tris-Acetat-EDTA)

Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA). 50X TAE BUFFER 1LElectrophoresis Buffer, storage temp: RT, 1000 ml

Thermo Scientific™ FastDigest™ Eco32I

Cut at GAT^ATC sites with Thermo Scientific™ FastDigest™ EcoRV (Eco32I), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: EcoRV). FASTDIGEST ECORV(ECO32I) 400UL

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