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Thermo Scientific™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 3 Peptide

Codice prodotto. 12020176
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Quantità:
800 x 20μL, 800 x 20 μL Assays
Dimensione della confezione:
800 reazioni
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Codice prodotto. Quantità Dimensione della confezione
12020176 800 x 20μL, 800 x 20 μL Assays 800 reazioni
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Codice prodotto. 12020176 Fornitore Thermo Scientific™ N. del fornitore PV3176
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Verfügbar ab: 01-12-2026
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Descrizione

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format.

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon bovine MBP Thr-97 derived peptide, containing a P-X-(S/T)-P consensus sequence, where X is any residue.

TRUSTED_SUSTAINABILITY

Specifica

Descrizione Z'-LYTE™ Kinase Assay Kit - Ser/Thr 3 Peptide, 800 x 20μL Assays, 800 rxns, 405/465nm Excitation, 535nm Emission, Methylcoumarin Label, FRET Technique, Serine/Threonine Kinases Target Enzyme, Peptide-Based Substrate Properties, End Point Readout, Used For Pharma and Biopharma, Protein Assays and Analysis
Product Line Z ́-LYTE
Da utilizzare con (applicazione) Pharma and biopharma, protein assays and analysis, target and lead identification and validation, FRET
Include All necessary reagents for performing primary or secondary screens
Volume del campione 800 x 20μL
Content And Storage –80°C, Z'-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
Saggio Serine/Threonine Kinase Assay
Assay Entry Biochemical phospho-specific activity (no antibody)
Product Type Z'-LYTE™ Kinase Assay Kit - Ser/Thr 3 Peptide
Quantità 800 x 20μL, 800 x 20 μL Assays
Formato 384-well plate
Excitation/Emission 405/465, 535 nm
Label or Dye Methylcoumarin
N. di reazioni 800 x 20 μL Reactions
Readout End Point
References BBRC vol. 61, pp 559-563 (1974), JBC, vol 252, p 4888 (1978)
Shipping Condition Dry Ice
Sorgente Bovine MBP, Thr-97 Derived Peptide S5, Consensus Seq Pro-X-(Ser⁄Thr)-Pro (APRTPGGRR)
Substrate Properties Peptide-Based Substrate
Target Entry MAPK13, MAP2K1, MAP2K6, MAPK1, MAP3K8, BRAF, MAP2K2, MAPK12, MAPK3, BRAF V599E
Target Enzyme Serine/Threonine Kinases
Validated Kinases BRAF, MAPK1, MAPK3, MAPK12, MAPK13, MAP2K1, MAP2K2, MAP3K6, MAP2K8
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For Research Use Only. Not for use in diagnostic procedures.

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