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Thermo Scientific™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 12 Peptide

Codice prodotto. 12040186
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Quantità:
800 x 20μL, 800 x 20 μL Assays
Dimensione della confezione:
800 reazioni
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Codice prodotto. Quantità Dimensione della confezione
12040186 800 x 20μL, 800 x 20 μL Assays 800 reazioni
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Codice prodotto. 12040186 Fornitore Thermo Scientific™ N. del fornitore PV3673
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Verfügbar ab: 01-12-2026
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Descrizione

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format.

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon CDK7, containing Ser-164 and a (S/T)-P-X-(K/R) consensus sequence, where X is any residue.

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Specifica

Descrizione Z'-LYTE™ Ser/Thr 12 Peptide Kinase Assay Kit, 800 x 20μL, 800rxns, Serine/Threonine Kinase Assay Type, Peptide-Based Substrate, 535nm Emission, 405/465nm Excitation, Methylcoumarin Label, End Point Readout, Serine/Threonine Kinases Target Enzyme, FRET Technique, Used for Pharma and Biopharma, Protein Assays and Analysis
Product Line Z ́-LYTE
Da utilizzare con (applicazione) Pharma and biopharma, protein biology, drug discovery and development, target and lead identification and validation, kinase biology, biochemical kinase assays, z and acutelyte™ kinase assays, enzyme and protein activity assays, protein assays and analysis, kinase activity, FRET
Include Z'-LYTE™ Ser/Thr 11 peptide, Z'-LYTE™ Ser/Thr 11 phospho-peptide, 5X kinase buffer A, ATP, development reagent B, development buffer
Volume del campione 800 x 20μL
Content And Storage –80°C, Z'-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
Saggio Serine/Threonine Kinase Assay
Assay Entry Biochemical phospho-specific activity (no antibody)
Product Type Z'-LYTE™ Ser/Thr 12 Peptide Kinase Assay Kit
Quantità 800 x 20μL, 800 x 20 μL Assays
Formato 384-well plate
Excitation/Emission 405/465, 535 nm
Label or Dye Methylcoumarin
N. di reazioni 800 Reactions
Readout End Point
References Mol. Cell. Biol. Vol 21. pp. 88-99 (2001)
Shipping Condition Dry Ice
Sorgente Based on CDK7 (GLAKSFGSPNRAY(pT)H)
Substrate Properties Peptide-Based Substrate
Target Entry CDK1, CDK2, CDK5
Target Enzyme Serine/Threonine Kinases
Validated Kinases CDK1, CDK2, CDK5
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For Research Use Only. Not for use in diagnostic procedures.

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