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  5. Invitrogen™ Human VWF ELISA Kit

Invitrogen™ Human VWF ELISA Kit

Codice prodotto. 15475173
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Quantità:
5 x 96 Tests
96 Tests
Dimensione della confezione:
1 set
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Codice prodotto. Quantità Dimensione della confezione
15475173 96 Tests 1 set
17185599 5 x 96 Tests Pezzo
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Codice prodotto. 15475173 Fornitore Invitrogen™ N. del fornitore EHVWF
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Descrizione

ELISA

The Human von Willebrand Factor (vWF) ELISA quantitates Hu vWF in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu vWF. Principle of the method The Human vWF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Von Willebrand factor (vWf) is a series of self-aggregated structures, all derived from common glycoprotein subunits, synthesized in endothelial cells and megakaryocytes. The molecular mass of the monomeric vWf unit is 225 kDa. vWf binds to circulating factor VIIIc (antihemophilic factor), hereby stabilizing the latter thus being an important factor in the normal primary haemostasis.
TRUSTED_SUSTAINABILITY

Specifica

Numero di accesso P04275
Intervallo saggio 0.123-30 ng/mL
Assay Sensitivity 0.13 ng/mL
Intervallo di calibrazione 0.4 ng/mL to 120 ng/mL
Content And Storage Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
Saggio Solid-phase sandwich ELISA
Coniugato HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Famiglia di proteine Other Proteins
Tipo di campione Plasma, Serum, Supernatant
Specificità This ELISA antibody pair detects human vWF. Other species not determined.
Sufficiente per 96 assays
Target Species Human
Da utilizzare con (applicazione) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Da utilizzare con (apparecchiatura) Absorbance Microplate Reader
Alias gene 6820430P06Rik; AI551257; B130011O06Rik; C630030D09; Coagulation Factor VIII; coagulation factor VIII VWF; EMBL:CAB37852.1}; F8VWF; factor viii related antigen; von Willebrand antigen 2; von Willebrand antigen II; von Willebrand Disease (vWD); von Willebrand factor; Von Willebrand factor homolog; VWD; vwf; vwf {ECO:00003126820430P06Rik; AI551257; B130011O06Rik; C630030D09; Coagulation Factor VIII; coagulation factor VIII VWF; EMBL:CAB37852.1}; F8VWF; factor viii related antigen; von Willebrand antigen 2; von Willebrand antigen II; von Willebrand Disease (vWD); von Willebrand factor; Von Willebrand factor homolog; VWD; vwf; vwf {ECO:0000312; F8VWF; VWD
ID gene (immissione) 7450
Simbolo del gene VWF
Interassay CV <12%
Intraassay CV <10%
Contenuto kit Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Protein Aliases vWF, von Willebrand antigen II
Quantità 96 Tests
Status giuridico RUO
Disciplina di ricerca Angiogenesis
Volume del campione Plasma 2 μL, Serum 2 μL, Supernatant 100 μL
Shipping Condition Wet or Dry Ice
Requisiti di stoccaggio Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Sinonimo F8VWF, VWD
Bersaglio VWF
Durata del test 1 hr 20 min
Tempo totale saggio 4 hr 45 min
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