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Invitrogen™ Triiodothyronine (T3) Competitive ELISA Kit
Competitive ELISA
Marke: Invitrogen™ EIAT3C
Additional Details : Gewicht : 0.00100kg Transport : UN number : 1789 Chem class : 8 Pack group : II
Includes: Vorbeschichtete Platte(n), Standard, Nachweisantikörper, HRP-Konjugat, Assay-Puffer, Waschpuffer, Chromogen, Stopplösung, Plattenabdeckungen und chargenspezifisches technisches Datenblatt.
Beschreibung
The Triiodothyronine (T3) ELISA quantitates T3 in serum,plasma,urine,dried fecal extracts,or cell culture medium.
Principle of the method
The Triiodothyronine (T3) Competitive ELISA research-use-only kit is designed to quantitatively measure T3 present in extracted serum and plasma,urine,extracted dried fecal samples,and tissue culture media samples. This kit measures total T3 in extracted serum and plasma and in extracted fecal samples. A T3 stock solution is provided to generate standard curves for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A T3-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a sheep antibody to T3 to each well. After a two-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound T3-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.
Spezifikation
78.125 bis 5000 pg/ml | |
Kompetitives ELISA-Kit für Triiodothyronin (T3) | |
HRP | |
ELISA-Kit | |
Fäkalien Extrakt, Plasma, Serum, Überstand, Urin | |
Human | |
Kolorimetrisches Mikrotiterplatten-Lesegerät | |
6.3 % | |
HRP | |
RUO | |
Stuhlextrakt, 100 μl; Plasma, 100 μl; Serum, 100 μl; Überstand, 100 μl; Urin, 25 μl | |
Chemikalien | |
2 Std 30 Min |
37,4 pg/mL | |
Multiplex-Assay auf der Luminex Plattform | |
Fluoreszent | |
Schilddrüsenhormon | |
96 Assays | |
ELISA, Protein-Assays, Aktivitäts-Assays, Proteinbiologie, Reproduktive Biologie, Entzündungen, Oxidativer Stress, Metabolismus, Zellsignalweiterleitung, Epigenetik, Hämatologie, DNA-Schaden, Krebsbiologie, Immunologie, Neurowissenschaften, Neurobiologie, Steroid-Hormon-Nachweis | |
13.4 % | |
Vorbeschichtete 96-Well-Platte, Assay-Verdünnungskonzentrat, HRP-konjugierter Nachweisantikörper., Waschpuffer, Stopplösung, Dichtungsfolien für Mikrotiterplatten, Stopplösung, Dichtungsfolien für Mikrotiterplatten | |
96 Tests | |
Stoffwechsel | |
−20 °C | |
1 Std 20 Min |