Invitrogen™ Human MMP-13 Recombinant Protein

Beschreibung

MMP13 Recombinant Protein, RP-77530, from Invitrogen.

MMP13 (collagense 3) plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC, and ACAN. MMP13 cleaves tripal helical collagens, including type I, II, and III collagen - but has the highest activity with soluble type II collagen. It can degrade collagen type IV, XIV, and X. MMP13 plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization, and ossification. It is required for normal embryonic bone development and ossificaiton. Mutations affecting the gene can lead to spondyloepimetaphyseal dysplasia Missouri type, metaphyseal anadysplasia 1, and metaphyseal dysplasia Sphar type. RP-77530 is a purified recombinant MMP-13 protein, expressed in Sf9-insect cells using the baculovirus expression vector system. This protein contains the 452 amino acids of full-length Procollagenase-3, with a C-terminal His-tag. Upon incubation with APMA, active collagenase-3 is formed. RP-77530 may be used as a control with corresponding anti-MMP-13 antibodies. Specific Activity: The specific activity of collagenase-3 is >200 mU/mg, with 1 U defined as the activity that hydrolyzes 1 umol peptide within 1 minute in an enzyme assay.