missing translation for 'onlineSavingsMsg'
Learn More

TER-119 Rat anti-Mouse, Super Bright 645, Clone: TER-119, eBioscience™

Rat Monoclonal Antibody

Marke:  Invitrogen 64-5921-80

Additional Details : Gewicht : 0.01000kg

 Lernen Sie weitere Varianten dieses

Artikelnummer. 15963664

  • 166.00 CHF / 25 Mikrogramm


Voraussichtliches Versanddatum: 08-10-2021
Zum Warenkorb hinzufügen

Beschreibung

Beschreibung

Description: The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G, a 21-25 kDa protein also known as the myeloid differentiation antigen Gr-1. A GPI-linked protein, Gr-1 is expressed by the myeloid lineage in a developmentally regulated manner in the bone marrow. While monocytes only express Gr-1 transiently during their bone marrow development, the expression of Gr-1 on bone marrow granulocytes as well as on peripheral neutrophils is a good marker for these populations. eBioscience testing indicates that in the bone marrow and lysed whole blood, the antibody clone RB6-8C5 also stains cells that express the highest levels of Ly6c (as defined by staining with antibody clone HK1.4). It is recommended that 1A8-Ly6G (cat. 9668) be used when looking at Ly-6G specific targets. Applications Reported: This RB6-8C5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This RB6-8C5 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product No. SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. TER-119 is a lineage marker for erythroid cells from early proerythroblast to mature erythrocyte stages in adult blood, spleen, and bone marrow. It is also present in yolk sac, and fetal and newborn liver. The TER-119 antigen is not expressed by cells of earlier erythroid development at BFU-e (blast-forming unit erythroid) stage or CFU-e (colony-forming unit erythroid) stage.
Spezifikation

Spezifikation

TER-119
Monoclonal
0.2 mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
Liquid
Ly76
IgG2b, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
104231
Flow Cytometry
TER-119
Super Bright 645
Ly76
Erythroid cell marker; Ly-76; Ly76; TER119
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation
Zertifikate