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TE-Puffer, pH 8.0, RNAse-frei

Product Code. 15445059
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Product Code. 15445059 Supplier Thermo Scientific Alfa Aesar Supplier No. J62745.=E
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Description

CAS: 1185-53-1 | C4H12ClNO3 | 157.594 g/mol

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris-EDTA (TE) buffer solution, pH 8.0 may also be used as a washing buffer. TE buffer is often used to store DNA and RNA while protecting it from degradation. Some protocols use TE 10:0.1 with 0.1 mM EDTA to reduce the interaction of the EDTA with downstream applications.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Anwendungen
Trizma wird bei der Formulierung von Pufferlösungen im pH-Bereich zwischen 7.5 und 8.5 verwendet. Tris-Pufferlösungen werden in der Zell- und Molekularbiologie für Prozesse wie die Extraktion und Reinigung von Proteinen und Nukleinsäuren weit verbreitet eingesetzt. Tris-EDTA(TE)-Pufferlösung, pH 8.0, kann auch als Waschpuffer verwendet werden. Der TE-Puffer wird häufig zur Lagerung von DNA und RNA verwendet, während sie gleichzeitig vor Zersetzung geschützt wird. Einige Protokolle verwenden TE 10:0.1 mit 0.1 mM EDTA, um die Interaktion der EDTA mit Downstream-Prozessen zu reduzieren.

Löslichkeit
Nicht mischbar oder schwer in Wasser mischbar.

Hinweise
200 mM Tris-HCl mit 20 mM EDTA
TRUSTED_SUSTAINABILITY

Chemical Identifiers

CAS 1185-53-1
Summenformel C4H12ClNO3
Molekulargewicht (g/mol) 157.594
MDL-Nummer MFCD00236359
InChI-Schlüssel QKNYBSVHEMOAJP-UHFFFAOYSA-N
PubChem CID 93573
IUPAC-Name 2-Amino-2-(Hydroxymethyl)Propan-1,3-Diol;Hydrochlorid
SMILES C(C(CO)(CO)N)O.Cl

Specifications

Chemischer Name oder Material TE buffer
Namenshinweis RNAse free
pH 8.0
Physikalische Form Flüssigkeit
Menge 125 mL
Löslichkeitsinformationen Not miscible or difficult to mix in water.

RUO – Research Use Only

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