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TCR V alpha 3.2 Monoclonal Antibody (RR3-16), PerCP-eFluor™ 710, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Marke: Affymetrix eBioscience 46-5799-82
Additional Details : Gewicht : 0.25000kg
Beschreibung
Description: This RR3-16 monoclonal antibody reacts with the mouse T cell receptor (TCR) V alpha 3.2 chain. Composed of an alpha and beta chain, TCR specificity is typically determined by Va, Ja, Vb, Db, and Jb gene rearrangement. The RR3-16 antibody recognizes the V alpha 3.2 chain on T cells from mouse strains bearing the b (e.g., C57BL/6) or c haplotype (e.g., SJL, SWR, and NOD) in the Tcra gene complex. The V alpha 3.2 chain is absent in mice with the a (e.g., Balb/c, AKR, C3H) and d (e.g., DBA/1 and DBA/2) haplotypes. Studies demonstrate that the V alpha 3.2 TCR is more highly expressed on CD8+ T cells. Applications Reported: This RR3-16 antibody has been reported for use in flow cytometric analysis. Applications Tested: This RR3-16 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
TCR V alpha 3.2 Monoclonal antibody specifically detects TCR V alpha 3.2 in Mouse samples. It is validated for Flow Cytometry.Spezifikation
TCR V alpha 3.2 | |
Monoclonal | |
0.2 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
Rat | |
100 μg | |
Primary | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
RR3-16 | |
PerCP-eFluor 710 | |
TCR V alpha3.2; TCRV alpha 3.2; TCRV alpha3.2; Va3.2; Valpha3.2 | |
Affinity chromatography | |
RUO | |
Mouse | |
Antibody | |
IgG2b κ |
For Research Use Only.