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Thermo Scientific™ Tango™ EDG1-bla U2OS Cells

Product Code. 10489228
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Menge:
2 x 106 Zellen
This item is not returnable. View return policy

Product Code. 10489228

Brand: Thermo Scientific™ K1520

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The Tango™ EDG1-bla U2OS cells contain the human Endothelial Differentiation, Sphingolipid G-protein-coupled Receptor, 1 (EDG1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.

The Tango™ EDG1-bla U2OS cells contain the human Endothelial Differentiation, Sphingolipid G-protein-coupled Receptor, 1 (EDG1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ EDG1-bla U2OS cells cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ EDG1-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product.

TRUSTED_SUSTAINABILITY

Specifications

Zugriffsnummer NM_001400.2
Assay-Eingabe Zellbasierte Beta-Arrestin-Rekrutierung
Zellenzustand Teilende Zellen
Inhalt und Lagerung K1520 Tango™ EDG1-bla U2OS-Zellen
(LiveBLAzer™-FRET B⁄G Lade-Kit, Lösung D und andere Materialien sind separat erforderlich; siehe Protokoll).
Lieferumfang:
• Tango™ EDG1-bla U2OS-Zellen
• Tango™ EDG1-bla U2OS-Assay-Protokoll
• Analysenzertifikat

Lagerung:
Die Tango™ EDG1-bla U2OS-Zellen werden auf Trockeneis geliefert und sollten sofort nach Erhalt in flüssigem Stickstoff gelagert werden.
Zytoplasmatischer Schwanz Nativer Schwanz
Nachweisverfahren Fluoreszent
Zur Verwendung mit (Anwendung) GPCRs
Gensymbol EDG1
Enthält EDG1-bla U2OS-Zellen, EDG1-bla U2OS-Assay-Protokoll, Analysenzertifikat
Populationsstatus Auf Einzelzellen basierende Klone
Produktlinie Tango
Menge 2 x 106 Zellen
Reporter-Gen BLA (Beta-Lactamase)
Zieleintrag EDG1
Typ U2OS-Zelle
Zelllinie U2OS
System Type Tango™
Druggable Target GPCRs
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Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

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