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  7. Invitrogen™ SuperScript™ IV One-Step RT-PCR System

Invitrogen™ SuperScript™ IV One-Step RT-PCR System

Codice prodotto. 15781999
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Codice prodotto. Include N. di reazioni Dimensione della confezione
15781999 Kit only 100 Reactions Pezzo
15814182 Kit only 25 Reactions 25 reazioni
15895891 Kit with ezDNase 25 Reactions 25 reazioni
15850233 Kit with ezDNase 100 Reactions 100 reazioni
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Codice prodotto. 15781999 Fornitore Invitrogen™ N. del fornitore 12594100
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Descrizione

Includes

  • SuperScript IV RT Mix, 12.5µL
  • 2X Platinum SuperFi RT-PCR Master Mix, 625µL
  • Nuclease-free water, 1.25mL

The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance.

The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance. With this convenient one-step formulation plus gene-specific primers, you can perform both cDNA synthesis from total RNA or mRNA and PCR amplification in a single tube.

Features of the SuperScript IV One-Step RT-PCR System include:

  • Superior performance with sensitivity down to 0.01 pg RNA, target length up to 13.8 kb, and the fastest one-step RT-PCR protocol
  • Reliable target detection even with suboptimally pure RNA samples
  • Two-phase hot-start activation mechanism for high sensitivity, specificity, and room temperature setup
  • Fast and easy gDNA removal for superior accuracy and confidence in results (SuperScript IV One-Step RT-PCR System with ezDNase only)

SuperScript IV RT is a proprietary MMLV RT mutant with increased processivity, thermostability, and inhibitor resistance, as well as reduced RNase H activity, for sensitive and efficient full-length cDNA synthesis. Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR. The combination of these two engineered enzymes produces enhanced RT-PCR yields and fidelity, as well as amplification of longer templates compared to other one-step RT-PCR kits.

The inclusion of ezDNase (SuperScript IV One-Step RT-PCR System with ezDNase only) for use upstream of the RT step further enhances the one-step RT-PCR workflow through an extremely simplified genomic DNA removal step that ensures the highest accuracy and confidence in one-step RT-PCR results.

TRUSTED_SUSTAINABILITY

Specifica

Content And Storage

• SuperScript IV RT Mix (50 μL)
• 2X Platinum SuperFi RT-PCR Master Mix (2 x 1.25 mL)
• Nuclease-free water (2 x 1.25 mL)

Store at –20°C.
Metodo di rilevamento Gel Electrophoresis
Formato Kit
GC-Rich PCR Performance High
PCR Method 1-step RT-PCR
Polimerasi Platinum SuperFi
Reaction Speed 10 min.
Tecnica 1-step RT-PCR
Optimal Reaction Temperature 45°C to 60°C
Quantità 1 Ea.
Reverse Transcriptase SuperScript IV
Ribonuclease H Activity Reduced
Shipping Condition Dry Ice
Tipo di prodotto finale PCR Amplified cDNA
Hot Start Built-In Hot Start
N. di reazioni 100 Reactions
Reaction Format Master Mix
Tipo di reagente Reverse Transcription
Dimensioni (prodotto finale) Up to 14 kb
Starting Material RNA
Include Kit only
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For Research Use Only. Not for use in diagnostic procedures.

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