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SK-BR-3 (human breast adenocarcinoma) Whole cell lysate, Denatured; Abnova
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Menge:
200 μg
Packungsgröße:
200 Mikrogramm
Beschreibung
Western Blotting
Spezifikation
Spezifikation
| Zur Verwendung mit (Anwendung) | Western Blot |
| Tissue | Breast |
| Beschreibung | Whole cell lysate (denatured) |
| Lysepuffer | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. |
| Vorbereitungsmethode | Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was mixed in 5X Sample Buffer to become final 1X Sample Buffer as Storage Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly. |
| Qualitätskontrollen | 12.5% SDS-PAGE Stained with Coomassie Blue. |
| Menge | 200 μg |
| Lagerungspuffer | In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue). |
| Wirtsspezies | Human |
| Konzentration | 3 mg/mL |
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