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Applied Biosystems™ TaqMan™ Assays zur SNP-Genotypisierung, menschlich
Alle ansehen Applied Biosystems ProdukteBeschreibung
- Unsere große Auswahl an SNP-Assays verbessert Ihre Validierungs- und Screening-Untersuchungen zu Anwendungen für Krankheitsassoziationen und Populationsstratifizierung
- Anwenderfreundliches Einzelröhrchenformat
- Das stabile Assay-Design liefert hochgenaue, reproduzierbare und zuverlässige Ergebnisse
- Ein einfacher Arbeitsablauf liefert schnelle Ergebnisse
Our predesigned TaqMan SNP Genotyping Human Assays are a genome-wide collection of millions of human assays, including common 1,000 Genome SNPs, HapMap SNPs, and coding SNPs.
Benefits:
- Proven—gold-standard TaqMan chemistry and robust assay designs deliver accurate, reproducible, and reliable results
- Easy—convenient single-tube format and simple workflow provide an easy path to trusted results; no optimization required
- Relevant—extensive collection of predesigned human assays offers direct access to content that is relevant to your research
- Tested—all human SNP genotyping assays ordered with VIC/FAM dye labels are functionally tested to ensure allelic discrimination. Assays ordered with Cyanine 5/ABY dyes are not functionally tested.
- Multiplexing ready—TaqMan assays are now available with with VIC/FAM or Cyanine 5/ABY formats to support simultaneous detection of two sets of SNPs (two pairs of alleles).
Approximate ship time
4–6 days in North America and 6–10 days in Europe (VIC/FAM); 4-10 days in North America, 6-14 days in Europe (Cyanine 5/ABY)
TaqMan SNP Genotyping Assays require only three reaction components for PCR: purified genomic DNA (1–20 ng), the assay solution, and TaqMan Genotyping Master Mix (singleplex) or TaqPath ProAmp Master Mix (multiplex) (sold separately).
All assay designs are the product of our bioinformatics pipeline, optimized over the course of more than a decade by leveraging manufacturing and assay performance data. TaqMan Assays have been cited in over 40,000 publications and are backed by more than 350 patents.
All of our predesigned TaqMan Assays are covered by the TaqMan Assays qPCR Guarantee.*
Recommended master mix (sold separately): TaqMan Genotyping Master Mix (Cat. No. 4371355) or TaqPath ProAmp Master Mix (Cat. No. A30865)
*Terms and conditions apply. For complete details, go to www.thermofisher.com/taqmanguarantee.
Spezifikation
Spezifikation
| Konzentration | 80X |
| Inhalt und Lagerung | •1 tube containing a 40X (S and M sizes) or 80X (L size) mix of pre-formulated assay (2 probes and 2 primers). •Store at -15°C to -25°C. |
| Nachweisverfahren | qPCR |
| Form | Liquid |
| Format | Tube |
| PCR-Methode | qPCR |
| Reaktionsgeschwindigkeit | Fast |
| Zur Verwendung mit (Geräte) | 7500 System, 7500 Fast System, 7900HT System, StepOne, StepOnePlus, ViiA 7 System, QuantStudio Absolute Q Digital PCR System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 12k Flex ProFlex PCR System*, VeritiPro*, SimpliAmp*, MiniAmp*, Automated Thermal Cycler** If a thermal cycler is used for PCR amplification, the optional pre-read and the post-read must be performed separately on a real-time PCR system in order to detect and record fluorescent signals. |
| Interne Sondenmodifikation | Cyanine 5 - MGB probe detects Allele 1, ABY - MGB probe detects Allele 2 |
| Marker oder Farbstoff | ABY, Cy5 |
| Mehr anzeigen |
Nur für Forschungszwecke. Darf nicht für diagnostische Verfahren eingesetzt werden.
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