Invitrogen™ Mouse SDF-1 alpha/CXCL12 ELISA Kit

Beschreibung

The Mouse SDF-1 alpha/CXCL12 ELISA quantitates Ms SDF-1α in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms SDF-1α. Principle of the method The Mouse SDF-1α solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CXCL12 ist ein von Stromazellen abgeleitetes alpha-Chemokin-Mitglied der Interkrin-Familie. Dieses Genprodukt und sein Rezeptor CXCR4 können Lymphozyten aktivieren und sind mit der Metastasierung einiger Krebsarten wie Brustkrebs assoziiert. Mutationen in diesem Gen sind mit einer Resistenz gegen Infektionen des humanen Immundefizienzvirus Typ 1 assoziiert. Für dieses Gen wurden mehrere Transkriptvarianten gefunden, die unterschiedliche Isoformen kodieren.