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enQuireBio™ Recombinant Human NAD-dependent malic enzyme, mitochondrial Protein

A DNA sequence encoding the Human NAD-dependent malic enzyme, mitochondrial, was expressed in the hosts and tags indicated.

Marke:  enQuireBio™ QP10156-ec-250ug

Additional Details : Gewicht : 0.01000kg

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Artikelnummer. 15982918

  • 2775.00 CHF / 250 Mikrogramm


Verfügbar ab
28-10-2020
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Spezifikation

Spezifikation

NP_001161807.1
NAD-dependent malic enzyme, mitochondrial Protein
250 μg
Less than 1.0 EU/ug as determined by LAL method.
Partial (see sequence information for more details).
Recombinant Protein
E. coli
MLHIKEKGKP LMLNPRTNKG MAFTLQERQM LGLQGLLPPK IETQDIQALR FHRNLKKMTS PLEKYIYIM GIQERNEKLF YRILQDDIES LMPIVYTPTV GLACSQYGHI FRRPKGLFIS ISDRGHVRS IVDNWPENHV KAVVVTDGER ILGLGDLGVY GMGIPVGKLC LYTACAGIRP DRCLPVCID VGTDNIALLK DPFYMGLYQK RDRTQQYDDL IDEFMKAITD RYGRNTLIQF EDFGNHNAF RFLRKYREKY CTFNDDIQGT AAVALAGLLA AQKVISKPIS EHKILFLGAG EAALGIANL IVMSMVENGL SEQEAQKKIW MFDKYGLLVK GRKAKIDSYQ EPFTHSAPES IPDTFEDAV NILKPSTIIG VAGAGRLFTP DVIRAMASIN ERPVIFALSN PTAQAECTAE EAYTLTEGR CLFASGSPFG PVKLTDGRVF TPGQGNNVYI FPGVALAVIL CNTRHISDSV FLEAAKALT SQLTDEELAQ GRLYPPLANI QEVSINIAIK VTEYLYANKM AFRYPEPEDK AKYVKERTW RSEYDSLLPD VYEWPESASS PPVITEHHHH HH>
4200
Greater than 95% as determined by SDS-PAGE and HPLC.
Research Use Only
ME2
Malic Enzyme activity was assayed spectrophotometrically at 340nm as described in Mandela and Sauer (1975). The standard reaction mixture contained 50 mM Tris-HCl, 3 mM MnCl2, 5 mM malate, 0.12 mM NADP+, 2.5 mM fumarate. Assay was performed in a Beckman spectrophotometer. The Km value is 1.5 ± 0.6 mM.
Human
His
0.2um filtered PBS, pH 7.4. , lyophilized
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