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enQuireBio™ Recombinant Human MEK1 Protein

A cDNA sequence encoding the MEK1 was constructed and used to recombinantly synthesize the protein.

Marke:  enQuireBio™ QP10784-1ug

Additional Details : Gewicht : 0.01000kg

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Artikelnummer. 15965158

Menge Preis
1 128.22 CHF / 1 Mikrogramm
10 117.77 CHF / 1 Mikrogramm
Voraussichtliches Versanddatum: 03-11-2021

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Beschreibung

Beschreibung

Spezifikation

Spezifikation

MEK1 Protein
1 μg
< 1.0 EU per ug protein as determined by the LAL method.
Recombinant Protein
Insect
MPKKKPTPIQ LNPAPDGSAV NGTSSAETNL EALQKKLEEL ELDEQQRKRL EAFLTQKQKV GELKDDDFEK ISELGAGNGG VVFKVSHKPS GLVMARKLIH LEIKPAIRNQ IIRELQVLHE CNSPYIVGFY GAFYSDGEIS ICMEHMDGGS LDQVLKKAGR IPEQILGKVS IAVIKGLTYL REKHKIMHRD VKPSNILVNS RGEIKLCDFG VSGQLIDSMA NSFVGTRSYM SPERLQGTHY SVQSDIWSMG LSLVEMAVGR YPIPPPDAKE LELMFGCQVE GDAAETPPRP RTPGRPLSSY GMDSRPPMAI FELLDYIVNE PPPKLPSGVF SLEFQDFVNK CLIKNPAERA DLKQLMVHAF IKRSDAEEVD FAGWLCSTIG LNQPSTPTHA AGV
Greater than 90% as determined by SDS-PAGE.
Research Use Only
∼125-175 units/mg. One unit of MEK1 activity transfers 1 nmol of phosphate to ERK1/2 peptide per minute at 30C in a reaction containing 100microm ATP. Recombinant active MEK1 also phosphorylates ERK1, ERK2, and GSK-3?. Kinase activity may vary depending on the substrate and reaction conditions. The optimal concentration should be determined for each specific application.
Human
His
MEK1 is supplied at a concentration of in 40mM Tris, pH 8.0, 0.15M NaCl, 0.27M sucrose, 1mM DTT, 0.2mM PMSF, 1mM benzamidine, 0.1mM sodium vanadate and 0.03% Brij-35.
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