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R&D Systems™ Recombinant Human B7-H2 Fc Chimera Protein
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity
Marke: R&D Systems™ 165-B7-100
Additional Details : Gewicht : 0.00970kg
Beschreibung
Measured by its ability to stimulate human T cell proliferation in the presence of anti-CD3. Wang, S. et al. (2000) Blood 96:2808. The ED50 for this effect is 0.3-1.2 μg/mL in the presence of anti-CD3 immobilized at least at 20 ng/mL. Optimal dilutions should be determined by each laboratory for each application.Spezifikation
| O75144 | |
| Lyophilized from a 0.2 μm filtered solution in PBS. | |
| M.W. (Observed): 80-90 kDa, reducing conditions; M.W. (theoretical): 53.4 kDa (monomer) | |
| 100 μg | |
| Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution. | |
| <0.01 EU / 1 μg of the protein by the LAL method. | |
| Unconjugated | |
| Reconstitute at 100 μg/mL in sterile PBS. | |
| B7-H2/ICOSLG |
| Bioactivity | |
| 23308 | |
| B7-H2 Fc Chimera | |
| Mouse myeloma cell line, NS0-derived human B7-H2 protein Human B7-H2 (Asp19-Ser258) (N-terminus) Accession # O75144 DIEGRMD Human IgG1 (Pro100-Lys330) (C-terminus) | |
| Disulfide-linked homodimer | |
| ICOSLG | |
| Recombinant | |
| >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining. |