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Rat IgG2a kappa, Super Bright 702, clone: eBR2a, Isotype Control, eBioscience™

Rat Isotype Control

Marke:  Invitrogen 67-4321-82

Additional Details : Gewicht : 0.01000kg

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Artikelnummer. 15815743

  • 365.00 CHF / 100 Mikrogramm


Geschätzter Ausliefertermin
von Anbieter 03-04-2019
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Beschreibung

Beschreibung

Description: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin. Applications Reported: APC-eFluor™ 780 Rat IgG2a Isotype Control has been reported for use in flow cytometric analysis. Applications Tested: Rat IgG2a K Isotype Control APC-eFluor™ 780 has been tested by flow cytometric analysis of mouse splenocytes. Use isotype control at the same concentration as experimental antibody.Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm. We recommend using a 710/50 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100μL of cell sample + 100μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 702 nm; Laser: Violet Laser
Spezifikation

Spezifikation

Rat IgG2a kappa
Control
0.2mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
IgG2, IgG2a, IgG2k
IgG2a, kappa
100μg
4°C, store in dark, DO NOT FREEZE!
Not Applicable
Control, Flow Cytometry
eBR2a
Super Bright 702
Liquid
Rat
Affinity chromatography
RUO
Isotype Control
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