Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 436, eBioscience™, Invitrogen™

Beschreibung

Description: The monoclonal rat IgG2a is useful as an isotype control immunoglobulin. Applications Reported: This eBR2a antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBR2a antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse splenocytes. Use the isotype control at the same concentration as the experimental antibody. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Rat IgG2a is an immunoglobulin subclass that is commonly used in research and diagnostic applications. It is produced by the rat immune system and is known for its ability to activate complement and mediate antibody-dependent cell-mediated cytotoxicity (ADCC). Rat IgG2a has a molecular weight of approximately 160 kDa and is composed of two heavy chains and two light chains. It is often used as a control or reference antibody in experiments involving rat models, and is also commonly used in flow cytometry and immunohistochemistry applications.