Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 660, eBioscience™, Invitrogen™

Description

Description: The monoclonal rat IgG2a is useful as an isotype control immunoglobulin. Applications Reported: eFluor® 660 Rat IgG2a Isotype Control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis. Applications Tested: eFluor® 660 Rat IgG2a Isotype Control has been tested by flow cytometric analysis of mouse splenocytes. This can be used at the same concentration as the experimental antibody. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

Rat IgG2a is an immunoglobulin subclass that is commonly used in research and diagnostic applications. It is produced by the rat immune system and is known for its ability to activate complement and mediate antibody-dependent cell-mediated cytotoxicity (ADCC). Rat IgG2a has a molecular weight of approximately 160 kDa and is composed of two heavy chains and two light chains. It is often used as a control or reference antibody in experiments involving rat models, and is also commonly used in flow cytometry and immunohistochemistry applications.