R&D Systems™ MagCellect Human CD4+ T Cell Isolation Kit

A mononuclear cell suspension is first incubated with the MagCellect Antibody Cocktail which targets the unwanted cells. MagCellect Streptavidin Ferrofluid is next added to the reaction which allows the streptavidin coated nanoparticles to interact with biotinylated antibody tagged cells. The tube containing the cell suspension is then placed within a magnetic field. Magnetically tagged cells will migrate toward the magnet (unwanted cell fraction), leaving the untagged cells or desired cell population in suspension to be harvested by aspiration while the tube remains in the magnetic field.

Designed for the isolation of cells in a liquid phase
Based on the use of Ferrofluids or magnetic nanoparticles that have no magnetic memory (superparamagnetic) and behave as colloids
Generates particles with higher ligand binding capacity per mass compared to many other larger diameter (>150nm) magnetic particles
The enriched cell preparation is then available for a variety of applications including tissue culture, immune status monitoring and flow cytometry