Thermo Scientific™ ProPac™ 3R SAX 3 μm HPLC Columns

Maximizing Reproducibility, Resolution, and Robustness

The ProPac 3R SAX 3 μm HPLC column is a strong anion-exchanger with a quaternary amine functional group, suitable for use in biomolecule applications. These specialized ion-exchange chromatography columns provide qualitative and quantitative evaluation of charge variant analysis to biopharmaceutical scientists who need reproducible resolving capabilities when identifying and quantifying charge variant profiles of proteins.

Our ProPac 3R SAX columns offer superior performance for characterization of proteins including monoclonal antibodies (mAbs), therapeutic proteins with acidic pI values, antibody drug conjugates (ADCs), biosimilar proteins, and adeno-associated viruses (AAV).

Column Features:

• Ideal for characterization and quality control assessment of closely-related protein variants
• Ability to separate full versus empty capsids for AAVs
• The high sample loading capability, without the carryover, allows for easy method optimization to provide you with excellent sensitivity and performance over a broad range of pH's, temperatures, and mobile phase compositions
• Compatible with mass spectrometry (MS)
• High-efficiency separations, resulting in a better understanding of charge variants in your samples
• Higher capacity than other columns based on nonporous particles
• Superior column-to-column and lot-to-lot reproducibility eliminates column variability as a concern in stability and QA/QC testing

We recommend our CX-1 pH gradient buffers when using these columns with LC-UV/LC-FLD.