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  6. Thermo Scientific™ POROS™ CaptureSelect™ AAVX Affinity Resin

Thermo Scientific™ POROS™ CaptureSelect™ AAVX Affinity Resin

Artikelnummer. 18486528 Alle ansehen Thermo Scientific Produkte
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Artikelnummer. 18486528 Lieferant Thermo Scientific™ Lieferanten-Nr. A36739

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Beschreibung

Thermo Scientific™ POROS™ CaptureSelect™ AAVX Affinity Resin is engineered to address the high selectivity and capacity requirements for large-scale downstream purification of a broad range of naturally occurring and synthetic adeno-associated virus (AAV) serotypes used for gene therapy applications.

POROS CaptureSelect AAVX Affinity Resin is engineered to address the high selectivity and capacity requirements for large-scale downstream purification of a broad range of naturally occurring and synthetic adeno-associated virus (AAV) serotypes used for gene therapy applications. POROS CaptureSelect AAVX resin has demonstrated binding reactivity towards a set of AAV serotypes that includes AAV1 to AAV8, and AAVrh10 as well as synthetic serotypes.

Features of POROS CaptureSelect AAVX resin include:

  • One-step AAV purification from crude material with high purity and yield
  • High specificity and capacity, maximizing yield by significantly reducing process steps
  • Superior capacity maintained at high flow rates
  • Excellent scalability and easier handling and packing due to rigid polymeric bead
  • Non-animal derived

POROS CaptureSelect AAVX Affinity Resin enables a robust, fast, and platformable purification process for wide-ranging AAV serotypes with excellent purity and yield obtained in one step. This resin comes with complete technical and regulatory support.

Main characteristics

  • Matrix: cross-linked poly(styrene-divinylbenzene) POROS beads
  • Average particle size: 50 μm
  • Ligand: CaptureSelect AAVX affinity ligand
  • Binding capacity: >1 x 1013 genome copies/mL of resin
  • Elution conditions: acidic elution with 0.1 M glycine, pH 3.0
  • Mechanical resistance: 100 bar (1450 psi; 10 MPa)
  • Formulation buffer: 18% (v/v) ethanol

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Spezifikation

Bindungsvermögen >1 x 1013 genome copies/mL of resin
Inhalt und Lagerung Store in refrigerator at 2°C to 8°C.
Versandbedingung Nasseis
Partikelgröße 50 μm
Form Flüssige Suspension
Produktlinie POROS
Menge 10 ml
Typ Harz-
Säulentyp Affinity
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