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Invitrogen™ Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit
Descrizione
The Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit enables optimal image-based analysis of four key markers of human pluripotent stem cells (PSCs): OCT4, SOX2, SSEA4, and TRA-1-60. This high performance immunocytochemistry (ICC) kit includes a complete set of primary and secondary antibodies, a nuclear DNA stain, and premade buffers for an optimized staining experiment. To characterize pluripotent stem cells using only two markers, we recommend us of the Pluripotent Stem Cell Immunocytochemistry Kit (OCT4, SSEA4) or the Pluripotent Stem Cell Immunocytochemistry Kit (SOX2, TRA-1-60).
With the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit, you can:
Confirm expression of key pluripotent stem cell markers with highly specific antibodies
Get more information per sample by measuring more than one marker at a time
Generate beautiful multiplexed images with minimal effort
Save time and avoid cell loss by minimizing unnecessary wash steps in your staining protocol
Process samples with confidence using a complete and optimized set of ICC reagents
Confirm Expression of Key PSC Markers
The antibodies included in the kit have been carefully selected and validated for high performance in the ICC analysis of PSCs, whether cultured under feeder-dependent or feeder-free media systems. OCT4 and SOX2 are critical nuclear markers, while SSEA4 and TRA-1-60 are important cell surface markers.
Get More Information Per Sample
The antibody combinations in the kit have been designed to allow for the specific and simultaneous assessment of two markers at a time (e.g., OCT4 & SSEA4 and SOX2 & TRA-1-60) along with nuclear DNA staining to help save time and precious sample.
Generate Beautiful Multiplexed Images
Powered by the superior performance of bright, photostable Alexa Fluor™ dyes, this kit enables the acquisition of beautiful multiplexed images of PSC marker expression using commonly available blue, green, and orange/red filter sets.
Minimize Unnecessary Wash Steps
The streamlined method recommended for this kit eliminates unnecessary wash steps found in standard ICC protocols to help save time and decrease the likelihood of cell loss during the staining protocol, especially when using glass coverslips or chamber slides. The only washes required are after incubation with primary and secondary antibodies.
Process Samples with Confidence
An optimized set of premade fixation, permeabilization, blocking, and wash buffers allows you to focus on answering scientific questions rather than spending time preparing reagents and determining compatibility.
What You Get
Easily scaled to any culture plate, dish, or slide format, the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit comes with reagents sufficient to stain 40 samples using a 50 uL staining volume. (See kit content details below.)
With the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit, you can:
Confirm Expression of Key PSC Markers
The antibodies included in the kit have been carefully selected and validated for high performance in the ICC analysis of PSCs, whether cultured under feeder-dependent or feeder-free media systems. OCT4 and SOX2 are critical nuclear markers, while SSEA4 and TRA-1-60 are important cell surface markers.
Get More Information Per Sample
The antibody combinations in the kit have been designed to allow for the specific and simultaneous assessment of two markers at a time (e.g., OCT4 & SSEA4 and SOX2 & TRA-1-60) along with nuclear DNA staining to help save time and precious sample.
Generate Beautiful Multiplexed Images
Powered by the superior performance of bright, photostable Alexa Fluor™ dyes, this kit enables the acquisition of beautiful multiplexed images of PSC marker expression using commonly available blue, green, and orange/red filter sets.
Minimize Unnecessary Wash Steps
The streamlined method recommended for this kit eliminates unnecessary wash steps found in standard ICC protocols to help save time and decrease the likelihood of cell loss during the staining protocol, especially when using glass coverslips or chamber slides. The only washes required are after incubation with primary and secondary antibodies.
Process Samples with Confidence
An optimized set of premade fixation, permeabilization, blocking, and wash buffers allows you to focus on answering scientific questions rather than spending time preparing reagents and determining compatibility.
What You Get
Easily scaled to any culture plate, dish, or slide format, the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit comes with reagents sufficient to stain 40 samples using a 50 uL staining volume. (See kit content details below.)
Specifica
Specifica
| Coniugato | 2 Color, Alexa Fluor 488, Alexa Fluor 555, Alexa Fluor 594 |
| Quantità | 40 tests |
| Content And Storage | Store at -20°C to 4°C: Store at -20°C to RT: |
| Product Type | Immunocytochemistry Kit |
Sicurezza e movimentazione
Identificativo prodotto
- Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit
- Pericolo
- cancerogenicità Categoria 1B
- Lesione oculare grave/irritazione oculare Categoria 1
- Mutagenicità di cellule germinali Categoria 2
- Sensibilizzazione della pelle Categoria 1
- Tossicità specifica per organi bersaglio (esposizione singola) Categoria 2
- H317-Può provocare una reazione allergica cutanea.
- H318-Provoca gravi lesioni oculari.
- H341-Sospettato di provocare alterazioni genetiche.
- H350-Può provocare il cancro.
- H371-Può provocare danni agli organi.
- P201-Procurarsi istruzioni specifiche prima dell'uso.
- P261-Evitare di respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.
- P264-Lavare accuratamente dopo l'uso.
- P270-Non mangiare, né bere, né fumare durante l'uso.
- P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
- P302+P352-IN CASO DI CONTATTO CON LA PELLE: lavare abbondantemente con acqua/.
- P305+P351+P338-IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.
- P308+P313-IN CASO di esposizione o di possibile esposizione, consultare un medico.
- P310-Contattare immediatamente un CENTRO ANTIVELENI/un medico .
- P332+P313-In caso di irritazione della pelle: consultare un medico.
- P501b-Smaltire il prodotto/recipiente in conformità con le disposizioni locali/regionali/nazionali/internazionali.
- MIXTURE LIST-Contain: Paraformaldehyde
For Research Use Only. Not for use in diagnostic procedures.
Titolo del prodotto
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