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Invitrogen™ Platinum™ Taq DNA Polymerase, DNA-free

Codice prodotto. 16315062
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2000 Reactions
400 Reactions
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pezzi 5
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Codice prodotto. 16315062 Fornitore Invitrogen™ N. del fornitore 15966005
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Invitrogen Platinum Taq DNA Polymerase, DNA-free, is manufactured using a novel, closed single-use system together with stringent quality-control testing to minimize the risk of contamination with bacterial and human DNA.

Invitrogen Platinum Taq DNA Polymerase, DNA-free, is manufactured using a novel, closed single-use system together with stringent quality-control testing to minimize the risk of contamination with bacterial and human DNA. This enzyme is an ideal choice for PCR-based applications requiring the high sensitivity without false-positive results from reagent-borne contamination. This DNA-free enzyme provides the same high level of performance and lot-to-lot consistency as standard Platinum Taq DNA Polymerase.

Platinum Taq DNA Polymerase, DNA-free, is a recombinant Taq polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures and dissociates after the initial denaturation step at 94°C. This automatic hot start provides increased sensitivity, specificity, and yield, while allowing convenient reaction assembly at room temperature. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase, DNA-free, has a non-template–dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5' to 3' exonuclease activity.

Features

  • Certified low DNA contamination level (≤0.01 copy of bacterial gDNA per enzyme unit)
  • Confidence in qualitative and quantitative detection of low-abundance microorganisms due to signal absence with no-template/reagents-only PCR controls
  • Same performance as Platinum Taq DNA Polymerase manufactured using conventional process

Applications

  • Amplification of DNA from complex genomic and viral templates
  • Hot start PCR
  • RT-PCR
TRUSTED_SUSTAINABILITY

Specifica

Concentrazione 5 U/μL
Content And Storage • Platinum Taq DNA Polymerase, DNA-free, 100 μL
• 10X PCR Buffer (without magnesium), DNA-free, 1.25 mL
• 50 mM MgCl2, DNA-free (1 mL)

Store at -20°C in a non-frost-free freezer.
Formato Tube
GC-Rich PCR Performance Low
Polimerasi Platinum Taq DNA Polymerase
Reaction Speed Standard
Product Type Taq DNA Polymerase
Quantità 500 Units
Shipping Condition Dry Ice
Da utilizzare con (applicazione) Hot-start PCR
Fedeltà (rispetto a taq) 1X
Hot Start Built-In Hot Start
N. di reazioni 400 Reactions
Sporgenza 3'-A
Reaction Format Separate Components
Dimensioni (prodotto finale) 5 kb or less
Starting Material DNA
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For Research Use Only. Not for use in diagnostic procedures.

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