Learn More
Invitrogen™ Platinum™ SuperFi II PCR Master Mixes
-
540.00 CHF
464.65 CHF / 100 Reaktionen
Sparen 75.35 CHF
13% Rabatt
MIGLIOR PREZZO in promozione! Usa il codice promo "27967" per avere il tuo prezzo promozionale.
Descrizione
Invitrogen Platinum SuperFi II Green PCR Master Mix (2X) contains a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup, as well as two tracking dyes for direct loading of PCR products on gels. Platinum SuperFi II DNA Polymerase (Cat. No. 12361010) is a proofreading DNA polymerase that combines superior fidelity with innovative SuperFi II Buffer, enabling universal primer annealing for the highest success in PCR. It is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.
Features of Platinum SuperFi II PCR Master Mix include:
- Exceptional >300X Taq fidelity
- Universal primer annealing at 60°C
- Superior specificity, sensitivity, and yields
- Robust amplification of difficult-to-amplify targets (e.g., those with suboptimal purity, ˃65% GC content, long PCR requirement)
Platinum SuperFi II DNA Polymerase is an engineered enzyme with high processivity and increased resistance to PCR inhibitors. It also enables fast-cycling protocols and amplification of long targets (up to 20 kb). Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation. This technology also enables reaction set up at room temperature and provides increased sensitivity and yield.
Due to the unique composition of the SuperFi II PCR buffer, the annealing temperature is 60°C for most primer pairs designed following the general design rules. Isostabilizing molecules in the buffer increase primer-template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize the annealing temperature for each primer pair. With Platinum SuperFi II DNA Polymerase, different PCR assays can be cycled together using the same protocol with a universal primer annealing temperature and an extension step selected for the longest fragment to be amplified.
Applications of Platinum SuperFi II DNA Polymerase:
- Ultra High-fidelity PCR
- Template generation for sequencing
- CRISPR gene-editing verification and characterization
- Amplification from low-input or suboptimal-purity DNA
- Cloning and construct generation
- Microbial genomics and microbiome research
- Linear template generation for in vitro transcription and recombinant protein expression
- Long-range PCR
- Challenging GC-rich and AT-rich target amplification
- Rare variant and genetic diversity studies
- Multiplex target amplification
- Synthetic biology and DNA assembly
- Site-directed mutagenesis
Platinum SuperFi II PCR Master Mix (12368010) is also available, which is the same master mix without the tracking dyes.
Specifica
Specifica
| Concentrazione | 2X |
| Content And Storage | • Platinum SuperFi II Green PCR Master Mix (2 x 1.25 mL) • Nuclease-free water (2 x 1.25 mL) Store at –20°C. |
| GC-Rich PCR Performance | High |
| Polimerasi | Platinum SuperFi II DNA Polymerase |
| Reaction Speed | Fast |
| Product Type | PCR Master Mix |
| Quantità | 100 reactions |
| Shipping Condition | Dry Ice |
| Da utilizzare con (applicazione) | Hot-start PCR, High-fidelity PCR |
| Fedeltà (rispetto a taq) | >300X |
| Vedi altri risultati |
Facendo clic su Invia, l'utente riconosce che potrebbe essere contattato da Fisher Scientific in merito al feedback fornito in questo modulo. Non condivideremo le vostre informazioni per altri scopi. Tutte le informazioni di contatto fornite saranno conservate in conformità con la nostra Politica sulla privacy. Informativa sulla privacy.