Invitrogen™ Platinum™ SuperFi II DNA Polymerase

Features of Platinum SuperFi II DNA Polymerase include:

• Exceptional >300X Taq fidelity
• Universal primer annealing at 60°C
• Superior specificity, sensitivity, and yields
• Robust amplification of difficult-to-amplify targets

Platinum SuperFi II DNA Polymerase is an engineered enzyme with high processivity and increased resistance to PCR inhibitors. It also enables fast-cycling protocols and amplification of long targets (up to 20 kb). Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation. This technology also enables reaction setup at room temperature and provides increased sensitivity and yield.

Isostabilizing molecules in the buffer increase primer-template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize the annealing temperature for each primer pair. With Platinum SuperFi II DNA Polymerase, different PCR assays can be cycled together using the same protocol with a universal primer annealing temperature and an extension step selected for the longest fragment to be amplified.

Applications:

• Ultra High-fidelity PCR
• Template generation for sequencing
• CRISPR gene-editing verification and characterization
• Amplification from low-input or suboptimal-purity DNA
• Cloning and construct generation
• Microbial genomics and microbiome research
• Linear template generation for in vitro transcription and recombinant protein expression
• Long-range PCR
• Challenging GC-rich and AT-rich target amplification
• Rare variant and genetic diversity studies
• Multiplex target amplification
• Synthetic biology and DNA assembly
• Site-directed mutagenesis