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Invitrogen™ Platinum™ SuperFi™ DNA Polymerase

Codice prodotto. 15525033
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100 Reactions
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500 Reactions
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Codice prodotto. N. di reazioni Dimensione della confezione
15525033 100 Reactions 100 unità
15535033 500 Reactions 500 unità
15545033 5 x 500 Reactions 2500 unità
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Codice prodotto. 15525033 Fornitore Invitrogen™ N. del fornitore 12351010
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Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR.

Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR. Featuring >300X Taq fidelity, Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.

Platinum SuperFi DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased resistance to PCR inhibitors. This feature also enables fast-cycling protocols and amplification of long targets. The Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation.

Benefits of Platinum SuperFi DNA Polymerase

  • Exceptional >300X Taq fidelity
  • High specificity and increased yields with Platinum hot-start technology
  • Robust amplification of difficult targets including those of sub-optimal purity or high GC content
  • Room temperature reaction setup and 24 hour bench top stability of pre-assembled reactions
  • Amplification of samples with suboptimal purity

Applications

  • High-fidelity PCR
  • Cloning and sub-cloning
  • Site-directed mutagenesis
  • Amplification of GC-rich templates
  • Template generation for sequencing
  • High-throughput PCR
  • Long PCR

Notes

  • Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).
TRUSTED_SUSTAINABILITY

Specifica

Content And Storage • Platinum SuperFi DNA Polymerase (50 μL at 2 U/μL)
• 5X SuperFi Buffer (2 x 1.25 mL)
• 5X SuperFi GC Enhancer (1.25 mL)

Store at –5°C to –30°C.
GC-Rich PCR Performance High
Polimerasi Platinum SuperFi DNA Polymerase
Reaction Speed Fast
Quantità 100 units
Shipping Condition Wet or Dry Ice
Da utilizzare con (applicazione) Hot-start PCR, High-fidelity PCR
Fedeltà (rispetto a taq) 300X
Hot Start Built-In Hot Start
N. di reazioni 100 Reactions
Sporgenza Blunt
Reaction Format Separate Components
Dimensioni (prodotto finale) 20 kb or less
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For Research Use Only. Not for use in diagnostic procedures.

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