Invitrogen™ Platinum™ Direct PCR Universal Master Mix

Features of Platinum Direct PCR Universal Master Mix include:

• Direct DNA amplification from various samples
• Universal primer annealing at 60°C
• Optional Platinum GC Enhancer for specific amplification and improved yields of GC-rich targets
• Superior specificity, sensitivity, and yields

Platinum Direct PCR Universal Master Mix is designed to work with a variety of samples of different origins such as animal, human, plant, insect, worm, and bacterial samples. The quick lysis protocol enables efficient amplification from templates up to 8 kb in size and co-cycling of different length fragments.

Engineered Platinum II Taq DNA polymerase confers faster cycling and tolerance to reaction inhibitors originating from sample material. Proprietary Platinum Taq antibodies block polymerase activity at ambient temperatures and dissociate after the initial denaturation step. This automatic 'hot start' provides increased sensitivity, specificity, and yield, while allowing reaction assembly at room temperature.

Due to the unique composition of the Platinum Direct PCR Universal Master Mix, the annealing temperature of 60°C can be used for most primer pairs that follow the general design rules. Isostabilizing molecules in the buffer increase primer-template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize annealing temperature for each primer pair. Different PCR assays can be cycled together using the same protocol with a universal primer annealing temperature and an extension step selected for the longest fragment to be amplified.

Applications:

• Direct PCR from animal, plant and bacterial samples
• Genotyping
• GMO screening
• Food and environmental testing
• CRISPR gene-editing screening
• Target DNA detection from limited biological material
• Direct real-time PCR from cell lysates
• 16S rRNA gene amplification for microbial analysis
• Colony screening
• Target amplification from FFPE tissue