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  5. Thermo Scientific Chemicals PIPES, 0.5 M Pufferlösung, pH 7.5

Thermo Scientific Chemicals PIPES, 0.5 M Pufferlösung, pH 7.5

Artikelnummer. 15425809
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Product Code. 15425809 Supplier Thermo Scientific Chemicals Supplier No. J63617.AE
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Description

CAS: 5625-37-6 | C8H18N2O6S2 | 302.36 g/mol

PIPES is frequently used as a buffering agent in biochemistry. PIPES has pKa near the physiological pH which makes it useful in cell culture work. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues and has a negligible capacity to bind divalent ions. Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer is done.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Anwendungen
PIPES kommt häufig als Pufferlösung in der Biochemie zum Einsatz. PIPES hat pKa in der Nähe des physiologischen pH-Wertes, was es für die Zellkulturarbeit nützlich macht. PIPES minimieren den Lipidverlust beim Puffern der Glutaraldehydhistologie in Pflanzen- und Tiergeweben und haben eine vernachlässigbare Fähigkeit, zweiwertige Ionen zu binden. Die Fixierung von Pilzzoosporen für Fluoreszenzmikroskopie und Elektronenmikroskopie war optimal bei einer Kombination aus Glutaraldehyd und Formaldehyd in PIPES-Puffer.

Löslichkeit
Löslich in 1 M NaOH (0,5 M bei 20 °C), Wasser (∼1 g/l bei 100 °C) und 0,5 N NaOH (0,1 M).

Hinweis
Kühl lagern. Behälter an einem trockenen und gut belüfteten Ort dicht verschlossen halten. Von starken Oxidationsmitteln fernhalten.
TRUSTED_SUSTAINABILITY

Chemical Identifiers

CAS 5625-37-6
Summenformel C8H18N2O6S2
Molekulargewicht (g/mol) 302.36
MDL-Nummer MFCD00006159
InChI-Schlüssel IHPYMWDTONKSCO-UHFFFAOYSA-N
PubChem CID 79723
ChEBI CHEBI:44933
IUPAC-Name 2-[4-(2-sulfoethyl)Piperazin-1-yl]-Ethansulfonsäure
SMILES C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O

Specifications

Chemischer Name oder Material PIPES buffer soln.
Farbe Farblos
Konzentration 0.5 M
pH 7.5
Physikalische Form Flüssigkeit
Menge 100 mL
Löslichkeitsinformationen Soluble in 1M NaOH (0.5 M at 20°C),water (∽1g/L at 100°C),and 0.5 N NaOH (0.1 M).

RUO – Research Use Only

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