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Thermo Scientific™ High Select™ Phosphopeptide Enrichment Kits & Reagents
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Mass spectrometry (MS) is a key tool for identifying sites of protein phosphorylation and quantifying phosphorylation changes. However, MS analysis of protein phosphorylation is challenging due to the low stoichiometry, high hydrophilicity, poor ionization, and incomplete fragmentation of phosphopeptides. Given the low relative abundance of phosphorylation modifications in complex protein samples, enrichment is essential for successful MS analysis of phosphopeptides. High-Select Fe-NTA and TiO2 phosphopeptide enrichment kits complement our lysis, reduction, alkylation, and digestion reagents, along with our C18, graphite spin, and high-pH reversed-phase peptide fractionation columns to provide a complete workflow for phosphopeptide enrichment.
- Convenient—pre-formulated buffers for ease-of-use in spin-column (Fe-NTA and TiO2) or magnetic (Fe-NTA) formats to enable parallel processing of multiple samples
- High specificity—recovers phosphopeptides with > 90% selectivity
- Superior recovery—enriches more total and unique phosphopeptides than other commercially available resins
- High coverage—recovers peptides with single and multiple phosphorylation sites
- Scalable—compatible with automation, including the use of KingFisher magnetic particle processors
High-Select Fe-NTA Phosphopeptide Enrichment Kit enriches phosphopeptides from complex samples using iron-chelate resin in spin columns. Each column can enrich phosphopeptides from 0.5 to 5 mg of total protein digest as starting sample. Together with pre-formulated buffers, the improved and simplified procedure enriches up to 150 μg of phosphopeptides with greater than 90% selectivity in less than 45 minutes.
High-Select TiO2 Phosphopeptide Enrichment Kit includes 24 titanium dioxide (TiO2) spin tips with optimized buffers to facilitate preparation of enriched phosphopeptides. Each column can enrich phosphopeptides from 0.5 to 3 mg of total protein digest as starting sample. The optimized buffers and simplified procedure eliminate the need for pyrrolidine in the elution buffer and subsequent clean-up using graphite spin columns. Use of this kit results in enrichment of up to 150 μg of phosphopeptides with greater than 90% selectivity. TiO2 enriches a unique set of phosphopeptides that complements the High-Select Fe-NTA IMAC Phosphopeptide Enrichment Kit.
For high resolution analysis of the enriched phosphopeptides, the recommended LC column for Nanospray Flex source is Acclaim PepMap 100 C18 LC Column (Cat. No. 164942 or 164939). For EASY-Spray source, the recommended LC column is EASY-Spray C18 LC Column (Cat. No. ES902 or ES903). The stand-alone High Select Fe-NTA Magnetic Agarose beads provide flexibility for scale and throughput and may be used in both manual and automated platforms, including KingFisher 96 and KingFisher Flex magnetic particle processors.
Specifications
Specifications
| For Use With (Equipment) | Microcentrifuge, Mass Spectrometer |
| Product Type | TiO2 Phosphopeptide Enrichment Spin Tips |
| Product Line | Pierce |
| Content And Storage | Store at 4°C. |
| Starting Material | Peptides, Protease-digested Protein |
| Final Product Type | Peptides |
| Detection Method | Mass Spectrometry |
| Format | Spin Column |
| Workflow Step | Sample Enrichment |
| Quantity | 96 Tips |
Frequently Asked Questions (FAQs)
No. It is important to enrich with the TiO2 kit (Cat. No. A32993) first. Afterwards, the flow-through and wash fractions from this enrichment can be processed with the Fe-NTA kit (Cat. No. A32992). If this order is reversed (that is, Fe-NTA before TiO2), there will be 2 consequences as follows: 1. There will not be any significant additional recovery of peptides (maybe just a few more peptides). 2. There will be no enrichment for the multiple phosphorylated peptides, so those would be lost.
The two phosphopeptides enrichment kits, Fe-NTA and TiO2, enrich a complementary set of phosphopeptides. Our R&D has developed a Sequential enrichment of Metal Oxide Affinity Chromatography (see https://assets.thermofisher.com/TFS-Assets/CMD/posters/PO-65032-SMOAC-Phosphoproteomics-Peptides-ASMS2017-PO65032-EN.pdf and https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/10/High-SelectTM-SMOAC-Protocol.pdf?CID=bid_mic_r04_jp_cp0000_pjt0000_bid00000_0so_blg_protein_analysis_mass_spectrometry_bid_ts_mbr_24065_Social_LAB) where flow-through and wash fractions from TiO2 enrichment were combined and subjected to Fe-NTA enrichment. This sequential enrichment provides impressive coverage of phosphoproteomes.
There are four differences between the Fe-NTA kit (Cat. No. 88300) and the new High-Select Fe-NTA kit (Cat. No. A32992) kit as follows: 1. The selectivity - the ratio of number of phosphopeptides over total peptides - was significantly improved to 99% with Cat. No. A32992, because the reagents were extensively optimized for the phosphopeptide selection. 2. The phosphopeptide yield was also increased to 33 µg based on quantitative colorimetric peptide assay (Cat. No. 23275). 3. The reagent is a pre-formulated format, so mixing reagent to prepare the working solution from stock solutions provided in the old kit (Cat. No. 88300) is not necessary, so it is easier to handle. 4. The enrichment protocol is optimized and streamlined, which means there are many fewer steps than with Cat. No. 88300. Thus, it takes <45 min to finish the entire protocol compared to 2 hours with the old kit (Cat. No. 88300).
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