Invitrogen™ pHrodo™ Red and Green AM Intracellular pH Indicator Dyes

Both pHrodo Red and pHrodo Green pH indicator dyes are photostable and appropriate for use in multiplexing experiments. pHrodo Red easily multiplexes with a wide variety of blue, green, and far red dyes and reporters such as GFP, Fuo-4, calcein, NucBlue, CellEvent Caspase 3/7 green, Mitosox Green, and Mitotracker Deep Red, among many others. pHrodo Green easily multiplexes with a wide variety of blue, red, and far red dyes reporters such as Mitosox Red, CellEvent Caspase 3/7 Red, NucBlue, RFPs, and Mitotracker Deep Red, among many others.

The pHrodo Red and Green dyes have been modified with AM ester groups, resulting in an uncharged molecule that can permeate cell membranes. Once inside the cell, the lipophilic blocking groups of the dyes are cleaved by nonspecific esterases, resulting in compounds that are retained within the intracellular space. The fluorescence intensity of the probe then becomes an indicator of intracellular pH. Subsequent use of the Intracellular pH Calibration Buffer Kit (Cat. No. P35379) allows this intracellular pH to be quantified.

pHrodo Red and Green AM intracellular pH indicator dyes are compatible with various platforms, such as traditional fluorescence microscopy, high content screening (HCS), flow cytometry, and microplate-based fluorimetry or high throughput screening (HTS). These reagents are also compatible with widefield or confocal fluorescence microscopy, flow cytometry, fluorescence plate readers, and high content instruments.