Tocris Bioscience™ PA Janelia Fluor 549, SE

Key information: PA Janelia Fluor™ 549, SE is a yellow photoactivatable fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live and fixed cell imaging. Application: Suitable for single molecule tracking and super resolution microscopy (SRM) in live cells, specifically sptPALM (live cells) and PALM (fixed cells) techniques. Properties and Photophysical Data: Non-fluorescent until activated at 365 nm. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag™ and SNAP-tag™. Can be multiplexed with PA Janelia Fluor™ 646, SE (Cat. No. 6150) to perform two-color sptPALM in live cells with activation at 405 nm. Photochemical quantum yield of uncaging = 2.2%, with improved yield upon protein conjugation. Excitation and emission maxima (λ) are 551 - 553 nm and 570 - 573 nm, respectively. Please see the protocol for further information on protein/antibody labeling and conjugation. HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.