OPTIZYME™ ApaI, Fisher BioReagents™

Name: OPTIZYME&trade; ApaI, Fisher BioReagents&trade;
Brand: Fisher Bioreagents
SKU: 11924792
Price: 85.00 EUR

Marke: Fisher Bioreagents BP8025-1

Additional Details : Gewicht : 0.01000kg

Artikelnummer. 11924792

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Beschreibung

5'...G G G C C^C...3'
3'...C^C C G G G...5'

Supplied with: 10X OPTIZYME Buffer 1
Conditions for 100% Activity:

1X OPTIZYME Buffer 1: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl₂ and 0.1mg/ml BSA
Incubate at 37°C

Enzyme Activity in OPTIZYME buffers:

Buffer 1: 100%
Buffer 2: 20 - 50%
Buffer 3: 0 - 20%
Buffer 4: 20 - 50%
Buffer 5: 0 - 20%

Storage Buffer:

10mM Tris-HCl (pH 7.4 at 25°C), 50mM NaCl, 1 mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol

Ligation and Re-cleavage:

More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with ApaI.

Digestion of Agarose-embedded DNA:

A minimum of 5 units of ApaI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.

Note:

Incubation at 30°C results in a 2-fold increase in activity.
ApaI is inhibited by salt concentrations above 50 mM.
ApaI cleavage is impaired by overlapping dcm methylation. To avoid dcm methylation, use a dam-, dcm- strain.

Compatible Ends: BseSI, Eco24I, SduI
Methylation Effects:
Dam: Never overlaps - no effect
Dcm: May overlap - cleavage impaired

5'...GGGCCm5CW GG...3'

3'...CCCGG GWm5CC...5'

(Cleavage is impaired)

CpG: May overlap - cleavage impaired

5'...GGGCCm5C G...3'

3'...CCCGG Gm5C...5'

(Cleavage is impaired)

5'...m5C GGGCCm5C G...3'

3'... Gm5CCCGG Gm5C...5'

(Cleavage is impaired)

EcoKI: Never overlaps - no effect

EcoBI: Never overlaps - no effect

Spezifikation

Konzentration	10 U/μL
Inkubatortemperatur	37°C
Zur Verwendung mit (Anwendung)	>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with ApaI
Inhalt und Lagerung	Keep container tightly closed
Schnittstelle	GGGCC.C
Form	Liquid

Komponenten	25 to 50% Water, >50% Glycerin
pH	7.4
Lagerungspuffer	10mM Tris HCl (pH 7.4 at 25°C), 50mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Menge	5000 U
Produkttyp	Apal

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OPTIZYME™ ApaI, Fisher BioReagents™

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