Invitrogen™ Cytokine 25-Plex Human Panel

By measuring 25 analytes simultaneously, the assay panel the assay panel provides more data from each sample, saving costs and time. This assay may be run alone or in combination with other select Luminex singleplex kits. The panel uses magnetic beads, facilitating automation, decreasing hands-on time, and increasing throughput and precision. Suitable for use with Luminex 100™/200, FLEXMAP 3D™ and MAGPIX™ systems.

Comprehensive human analyte panel
The Human Cytokine Magnetic 25-Plex Panel for the Luminex platform provides reagents for the accurate, reproducible, and sensitive quantitation of human proteins including: GM-CSF, IFN-α, IL-10, IFN-γ, MCP-1, IL-1β, MIG, IL-1RA, MIP-1α, IL-2, MIP-1β, IL-2R, RANTES, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40/p70), IL-13, IL-15, IL-17, and TNF-α

Luminex xMAP™ technology—effective and proven analytical tool
The Human Cytokine Magnetic 25-Plex Panel is based on xMAP technology. The use of a suspension bead-based technology enables the multiplexing capabilities of the Luminex assays. Magnetic microspheres are internally dyed with red and infrared fluorophores of differing intensities. Each bead is given a unique number, or bead region, allowing differentiation of one bead from another. Beads covalently bound to different antibodies can be mixed in the same assay, using a 96-well microplate format. At the completion of the sandwich immunoassay, beads can be read using the Luminex 200, FLEXMAP 3D detection systems, instruments based on flow cytometry, two lasers, real-time digital signal processing, and xPONENT™ software to distinguish bead color (analyte) and assay signal strength (PE) fluorescence intensity.

MAGPIX™ system—brings the power of multiplex to every lab
The MAGPIX system may also be used for sample analysis. This instrument utilizes xMAP technology and is built on light-emitting diodes (LEDs) for excitation, a CCD camera for bead and analyte detection, and high-speed digital signal processors. The MAGPIX system is more compact and robust, providing a very cost effective magnetic bead-based multiplexing tool. Simple, out of the box set-up allows researchers to rapidly perform singleplex or multiplex assays of up to 50 targets in single biological samples. The MAGPIX system is easy to use—ideal for both new and experienced users alike.

Magnetic beads let you do more with your time
Magnetic bead-based assays use Luminex MagPlex magnetic microspheres. All other assay components are the same as the equivalent Luminex polystyrene bead-based assays providing identical quality and consistency performance. MagPlex technology takes advantage of magnetic properties to simplify assay wash steps and maximize uniformity of results. Magnetic bead-based assays also enable automation, decrease hands-on time, and increase throughput and precision. Easy-to-use protocols provide results in as little as 3.5 hours. In addition, these assays are compatible with both vacuum and magnetic wash stations and work well with the MAGPIX system as well as other Luminex xMAP platforms.

More validation for confidence in your results
The Human Cytokine Magnetic 25-Plex Panel has been validated using the same rigorous criteria that distinguishes Luminex assays Each kit supplied with a product insert that outlines the specifications for each marker in the assay.

• Superior performance: accurate, reproducible, and sensitive quantitation of multiple proteins
• High quality: fully qualified antibodies permit excellent specificity and sensitivity
• Fast and easy protocols: perform your multiplex assay and analyze your data typically in less than one day

Cell Analysis, Immunoassays, Ready-To-Use Immunoassay