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  5. Invitrogen™ Mouse IL-6 ELISA Kit 

Invitrogen™ Mouse IL-6 ELISA Kit

Codice prodotto. 10707833
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Quantità:
2 x 96 Tests
5 x 96 Tests
96 Tests
Dimensione della confezione:
Pezzo
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Codice prodotto. Quantità Dimensione della confezione
10707833 96 Tests Pezzo
10110623 2 x 96 Tests Pezzo
10476283 5 x 96 Tests Pezzo
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Codice prodotto. 10707833 Fornitore Invitrogen™ N. del fornitore KMC0061
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Descrizione

Includes

Mouse IL-6 Antibody Coated 96-Well Plate, Mouse IL-6 Standard, Standard Diluent Buffer, Mouse IL-6 Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer Concentrate (25X), Stabilized Chromogen, TMB, Stop Solution, Plate Covers, Detailed protocol with validation tests

ELISA

The Mouse Interleukin (IL-6)) ELISA quantitates Ms IL-6 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-6. Principle of the method The Mouse IL-6 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

Order Info

Shipping Condition: Wet ice

TRUSTED_SUSTAINABILITY

Specifica

Numero di accesso P08505
Intervallo saggio 7.8-500 pg/mL
Assay Sensitivity <3 pg/mL
Content And Storage Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
Coniugato HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Famiglia di proteine Cytokines & Receptors
Tipo di campione Plasma, Serum, Supernatant
Target Species Mouse
Da utilizzare con (applicazione) Colorimetric
Da utilizzare con (apparecchiatura) Absorbance Microplate Reader
Alias gene B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6; IL-6
ID gene (immissione) 16193
Simbolo del gene Il6
Interassay CV 6.7%
Intraassay CV 5.8%
Contenuto kit Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Precisione <3 pg/mL
Protein Aliases IL-6, B-cell hybridoma growth factor, Interleukin HP-1
Quantità 96 Tests
Status giuridico RUO
Volume del campione Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Shipping Condition Wet Ice
Requisiti di stoccaggio 2-8°C
Bersaglio IL-6
Durata del test 1 hr 20 min
Tempo totale saggio 3 hr 30 min
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For Research Use Only. Not for use in diagnostic procedures.

Titolo del prodotto
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