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Invitrogen™ Novex™ IFN-γ Mouse ELISA Kit, verbessert
Quantifiziert natürliche und rekombinante Maus-IFNγ in Serum, Plasma, gepufferter Lösung oder Zellkulturmedium
Marke: Invitrogen™ KMC4021
Additional Details : Gewicht : 0.70000kg
Includes: Vorbeschichtete Platte, Standard, Nachweisantikörper, Streptavidin-HRP, Verdünnungsmittel, Waschpuffer, Chromogen, Stopplösung und Plattenabdeckungen
Beschreibung
The Mouse Interferon Gamma (Ms IFNγ) ELISA quantitates Ms IFNγ in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IFNγ. Principle of the method The Mouse IFNγ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Interferon-gamma (IFN-γ) ist ein Lymphokin, das von aktivierten T- und NK-Zellen produziert wird.
Zellanalyse, Zellsignalweiterleitung, Zellviabilität, Zellproliferation und -funktion, Immunoassays, gebrauchsfertiger Immunoassay
Bestellinformation
Versandbedingung: Raumtemperatur
Spezifikation
P01580 | |
<2 pg/ml | |
ELISA-Kit | |
Maus | |
Kolorimetrisches Mikrotiterplatten-Lesegerät | |
IFN-g,Ifg | |
6.5 % | |
Vorbeschichtete 96-Well-Platte, Standard-Verdünnungspuffer, Biotinylierter Nachweisantikörper, HRP-Verdünnungsmittel, Waschpuffer, Stopplösung, Dichtungsfolien für Mikrotiterplatten, Chromogen, Stop Solution, Adhesive Plate Covers | |
IFN-γ | |
RUO | |
2 bis 8 °C | |
1 Std 20 Min |
47 bis 300 pg/ml | |
Biotin | |
Supernatant | |
Kolorimetrischer Nachweis | |
15978 | |
4 h | |
3.2 % | |
HRP | |
96 Assays | |
Plasma, 25 μl; Serum, 25 μl; Überstand, 25 μl | |
Maus | |
4 h |
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.