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Invitrogen™ eBioscience™ ELISA Ready-SET-Go!™ für Maus IL-17A (Homodimer) Kit

Speziell entwickelt für die genaue und präzise Messung des Gehalts an Maus-IL-17AA-Homodimer-Protein in Proben u.a. aus Serum und Zellkulturüberständen.

Marke:  Invitrogen™ 88-7371-88

Additional Details : Gewicht : 0.01000kg

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Artikelnummer. 12720170

  • 534.00 CHF / 1 Stück


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Includes: Capture-Antikörper: Vortitrierter, aufgereinigter Antikörper
Nachweisantikörper. Vortitrierter, biotinkonjugierter Antikörper
Standard. Rekombinantes Zytokin zur Erzeugung von Standardkurven und zur Kalibrierung von Proben
ELISA/ELISPOT Beschichtungspufferpulver. Dieses Ready-Set-Go! ELISA-Set kann ELISA/ELISPOT-Beschichtungspufferpulver (Rekonstituierbar bis 1 l mit dH20 und Filter mit 0.22 uM) oder 10x PBS ELISA-Beschichtungspuffer (Verdünnbar mit 1 Teil 10x-Puffer in 9 Teilen dH20) enthalten.
Assay-Verdünner. 5x konzentriert
Nachweisenzym Vortitrierte Avidin-HRP
Substratlösung. Tetramethylbenzidin (TMB)-Substratlösung

Beschreibung

Beschreibung

The Mouse Interleukin 17A Homodimer (IL-17A) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-17A. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Das ELISA Ready-SET-Go! Reagenzien-Set für Maus-IL-17A (mit oder ohne Mikrotiterplatten mit hoher Affinitätsbindung) enthält die erforderlichen Reagenzien, Puffer und Verdünnungsmittel für Enzyme-Linked Immunosorbent Assays (ELISA)
Spezifikation

Spezifikation

Q62386
ELISA-Kit
4-500 pg/mL
16171
RUO
2°C to 8°C
24 hr. 30 min.
Interleukin-17A, IL-17AA
Mouse
4 pg/mL
Colorimetric Microplate Reader
HRP
Biotin
ELISA
Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, ELISA/ELISPOT Coating Buffer Powder: This Uncoated ELISA Kitmay contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 µM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20)., Assay Diluent: 5X concentrated, Detection enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -44; -76; -86)
Serum, 100 μL|Supernatant, 100 μL
1 hr. 45 min.
Mouse
10 x 96 Tests
Supernatant
AV080368, Mcp-1
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation