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Mouse IgG2a kappa, Super Bright 645, Clone: eBM2a, Isotype Control, eBioscience™

Mouse Isotype Control

Marke:  Invitrogen 64-4724-82

Additional Details : Gewicht : 0.01000kg

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Artikelnummer. 15815693

  • 365.00 CHF / 100 Mikrogramm


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von Anbieter 04-03-2019
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Beschreibung

Beschreibung

Description: This is a monoclonal mouse IgG2a, kappa antibody. It is used as an isotype control for mouse IgG2a antibodies.Applications Reported: This mouse IgG2a isotype control has been reported for use in flow cytometric analysis.Applications Tested: This eBM2a antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells. This should be used at the same concentration as experimental antibody.Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100μL of cell sample + 100μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser
Spezifikation

Spezifikation

Mouse IgG2a kappa
Control
0.2mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
IgG2, IgG2a, IgG2ak
IgG2a, kappa
100μg
4°C, store in dark, DO NOT FREEZE!
Not Applicable
Control, Flow Cytometry
eBM2a
Super Bright 645
Liquid
Mouse
Affinity chromatography
RUO
Isotype Control
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For Research Use Only