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Invitrogen™ MCP-1/CCL2 Mouse Uncoated ELISA Kit

Uncoated ELISA Kit

Marke:  Invitrogen™ 88-7391-77

Additional Details : Gewicht : 0.01000kg

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Artikelnummer. 12740170

  • 768.00 CHF / 1 Stück


Verfügbar ab
19-07-2021
Zum Warenkorb hinzufügen

Includes: Capture-Antikörper: Vortitrierter, aufgereinigter Antikörper
Nachweisantikörper. Vortitrierter, biotinkonjugierter Antikörper
Standard. Rekombinantes Protein zur Erzeugung von Standardkurven und zur Kalibrierung von Proben
10X-Beschichtungspuffer. Puffer für die Beschichtung des Capture-Antikörpers
5x ELISA/ELISPOT-Verdünnungsmittel. Puffer zum Blockieren und Verdünnen des Nachweisantikörpers und -enzyms
Enzym. Vortitrierte Avidin-HRP
Substrat. 1x TMB-Lösung

Beschreibung

Beschreibung

The Mouse Monocyte Chemoattractant Protein-1/CCL2 (MCP-1) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse MCP-1. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Das ELISA Ready-SET-Go! Reagenzien-Set für Maus CCL2 (MCP-1) (mit oder ohne Mikrotiterplatten mit hoher Bindungsaffinität) enthält die erforderlichen Reagenzien, Puffer und Verdünnungsmittel für quantitative ELISAs (Enzym-linked Immunosorbent Assays)
Spezifikation

Spezifikation

P10148
ELISA-Kit
15-2,000 pg/mL
20296
RUO
2°C to 8°C
24 hr. 30 min.
Monocyte chemotactic protein 1
Mouse
15 pg/mL
Colorimetric Microplate Reader
HRP
Biotin
ELISA
Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -76; -86)
Plasma, 100 μL|Serum, 100 μL|Supernatant, 100 μL
1 hr. 45 min.
Mouse
20 x 96 Tests
Supernatant
AI553453, CCP-1/C11, CCP1, CTLA-1, CTLA1, GZB
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

For Research Use Only