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Invitrogen™ LIVE/DEAD™ Cell Imaging Kit (488/570)

Codice prodotto. 12353643
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Quantità:
1 mL kit
Dimensione della confezione:
Pezzo
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Codice prodotto. Quantità Dimensione della confezione
12353643 1 mL kit Pezzo
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Codice prodotto. 12353643 Fornitore Invitrogen™ N. del fornitore R37601
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    465.00 CHF
    371.65 CHF / 1 Milliliter
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Descrizione

Includes

Component A: 10 x 1mL vials; Component B: 1 x 10 vials, dried down

Sensitive two-color fluorescence cell viability assay optimized for FITC and Texas Red™ filters

  • Allows discrimination between live and dead cells with two probes that measure recognized parameters of cytotoxicity and cell viability: intracellular esterase activity and plasma membrane integrity
  • Based on cell-permeable dye for staining of live cells and cell-impermeable dye for staining of dead and dying cells
  • Adapted for imaging platforms by optimizing for common green and red imaging filters used with FITC and Texas Red
  • Live cell component produces intense, uniform green fluorescence in live cells (ex/em 488 nm/515nm
  • Dead cell component produces predominantly nuclear red fluorescence (ex/em 570nm/602nm) in cells with compromised cell membranes, strong indicator of cell death and cytotoxicity
  • Sensitive detection of key measures of cell health: viability, live/dead ratio, and cytotoxicity
  • Ease of use with minimal dilutions
  • Live cells are distinguished by presence of ubiquitous intracellular esterase activity as determined by the enzymatic conversion of virtually nonfluorescent cell-permeant calcein AM to intensely fluorescent calcein, which is well-retained within live cells
  • Red component is cell-impermeant and therefore only enters cells with damaged membranes
  • In dying and dead cells bright red fluorescence is generated upon binding to DNA
  • Background fluorescence levels are inherently low with this assay technique because dyes are virtually nonfluorescent before interacting with cells

Cell Analysis, Cell Counting and Viability, Cell Counting, Viability, and Cryopreservation, Cell Culture, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function

Order Info

Shipping Condition: Dry Ice

TRUSTED_SUSTAINABILITY

Specifica

Tipo di cellula Mammalian Cells
Colore Red, Green
Content And Storage • Component A: 10 x 1 mL vials
• Component B: 1 x 10 vials (dried down)

Store at -20°C
Descrizione LIVE/DEAD™ Cell Imaging Kit (488/570)
Metodo di rilevamento Fluorescence
Da utilizzare con (applicazione) Viability Assay
Da utilizzare con (apparecchiatura) Floid Cell Imaging System, Fluorescence Microscope
Product Type Cell Imaging Kit
Tipo di colorante Other Label(s) or Dye(s)
Forma Frozen
Formato Tube(s)
Tempo di incubazione 15 min
Product Line LIVE/DEAD, Molecular Probes
Quantità 1 mL kit
Shipping Condition Dry Ice
Eccitazione della lunghezza d'onda 488, 570 nm
Emissione lunghezza d'onda 515/602
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