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Invitrogen™ H2DCFDA (H2-DCF, DCF)

Codice prodotto. 11560326
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Quantità:
100 mg
Dimensione della confezione:
100mg
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Codice prodotto. Quantità Dimensione della confezione
11560326 100 mg 100mg
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Codice prodotto. 11560326 Fornitore Invitrogen™ N. del fornitore D399
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Descrizione

Chemically reduced form of fluorescein used as an indicator for reactive oxygen species in cells

The cell-permeant 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) (also known as dichlorofluores cin diacetate) is a chemically reduced form of fluorescein used as an indicator for reactive oxygen species (ROS) in cells, for example to detect the generation of reactive oxygen intermediates in neutrophils and macrophages. Upon cleavage of the acetate groups by intracellular esterases and oxidation, the nonfluorescent H2DCFDA is converted to the highly fluorescent 2',7'-dichlorofluorescein (DCF). Versions of this indicator that have better cellular retention are available (Cat. No. C400), (Cat. No. C2938).

ROS Indicator Specifications:

  • Ex/Em: ∼492–495/517–527 nm
  • Product is air sensitive and should be stored under dry argon or nitrogen
  • Product may be dissolved in DMSO, DMF, or ethanol for use
  • Indicator is cell permeant (cell loading protocols are available in the literature)
  • Fluorescence can be monitored using a flow cytometer, fluorometer, microplate reader, or fluorescence microscope, using excitation sources and filters appropriate for fluorescein

Find More ROS Indicators

We offer an assortment of Molecular Probes™ products for the generation of reactive oxygen species (ROS), including singlet oxygen, superoxide, hydroxyl radical and various peroxide and hydroperoxides, as well as for their fluorometric detection in solution. Review Generating and Detecting Reactive Oxygen Species—Section 18.2 in the Molecular Probes™ Handbook for more information on these products.

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifica

Quantità 100 mg
Da utilizzare con (applicazione) ROS
Metodo di rilevamento Fluorescence
Product Type ROS Indicator
Excitation Wavelength Range 492 to 495 nm
Intervallo di emissione lunghezza d'onda 517 to 527 nm

For Research Use Only. Not for use in diagnostic procedures.

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