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  5. Invitrogen™ Kit ELISA de MIG/CXCL9 humain

Invitrogen™ Kit ELISA de MIG/CXCL9 humain

Code produit 15494963
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Quantité:
5 x 96 tests
96 dosages
Unit Size:
1 kit
1 pièce
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Product Code. Quantité Unit Size
15494963 96 dosages 1 kit
17135419 5 x 96 tests 1 pièce
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Product Code. 15494963 Supplier Invitrogen™ Supplier No. EHCXCL9
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Description

ELISA

The Human MIG/CXCL9 (Hu MIG) ELISA quantitates Hu MIG in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MIG. Principle of the method The Human MIG solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

MIG, also known as CXCL9, is a T-cell chemoattractant inducible by gamma interferon that is a member of the CXC chemokine family of cytokines.This gene is mapped to 4q21. It is noted that, although the best-described activities of the chemokines are as chemotactic factors, chemokines also have an effect on T-cell activation, angiogenesis, and HIV infection. While most CXC chemokines are chemotactic for neutrophils, MIG and INP10 are unusual and similar in being CXC chemokines that are chemotactic for lymphocytes and inactive in neutrophils.
TRUSTED_SUSTAINABILITY

Specifications

Numéro d’adhésion Q07325
Plage de dosage 8,23-6000 pg/ml
Sensibilité du dosage 20 pg/ml
Plage d’étalonnage 20 pg/ml à 6000 pg/ml
Contenu et stockage Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
Doser ELISA sandwich en phase solide
Conjugué Biotine
Format du kit d’immunoessai Sandwich ELISA Kit
Type de produit Kit ELISA
Famille de protéines Analyse GCPR
Type d’échantillon Sérum, Surnageant, Surnageant
Spécificité Ce kit ELISA ne montre aucune réactivité croisée avec les cytokines testées : angiogénine humaine, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 bêta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, leptine, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 bêta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-bêta, TIMP-1, TIMP-2, TNF-alpha, TNF-bêta, TPO, VEGF.
Suffisant pour 96 dosages
Espèces cibles Humaine
À utiliser avec (application) ELISA, dosage des protéines, biologie des protéines, cancérologie, immunologie, neuroscience, neurobiologie, détection des protéines, analyses protéiques
À utiliser avec (équipement) Lecteur de microplaques colorimétriques
Alias de gène BB139920; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 9; CMK; crg-10; CXC; CXC motif chemokine; C-X-C motif chemokine 9; C-X-C motif chemokine ligand 9; CXCL; CXCL9; gamma interferon-induced monokine; gamma-interferon-induced monokine; HuMIG; M119; Mig; monokine induced by gamma interferon; monokine induced by interferon gamma; monokine induced by interferon-gamma; MuMIG; protein m119; SCYB9; small inducible cytokine B subfamily (Cys-X-Cys), member 9; small inducible cytokine B9; small-inducible cytokine B9BB139920; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 9; CMK; crg-10; CXC; CXC motif chemokine; C-X-C motif chemokine 9; C-X-C motif chemokine ligand 9; CXCL; CXCL9; gamma interferon-induced monokine; gamma-interferon-induced monokine; HuMIG; M119; Mig; monokine induced by gamma interferon; monokine induced by interferon gamma; monokine induced by interferon-gamma; MuMIG; protein m119; SCYB9; small inducible cytokine B subfamily (Cys-X-Cys), member 9; small inducible cytokine B9; small-inducible cytokine B9; CMK; Humig; MIG; SCYB9; crg-10
Identification génétique (Entrez) 4283
Symbole de gène(s) CMK,Humig,MIG,SCYB9,crg-10
Coefficient de variation inter-dosage < 12 %
Coefficient de variation intra-dosage < 10 %
Contenus des kits Plaque à 96 puits pré-enduite, Étalon, Concentrat de diluant de dosage, Anticorps de détection biotinylé, Tampon de lavage, Chromogène, Solution d’arrêt, Couvercles de plaques adhésives, Couvercles de plaques adhésives
Marqueur ou colorant HRP
Quantité 96 dosages
État réglementaire RUO
Disciplines de recherche Inflammation
Volume d’échantillon Plasma, 50 μl ; sérum, 50 μl ; surnageant, 100 μl
Conditions d’expédition Wet or Dry Ice
Conditions de stockage 2°C à 8°C
Synonyme CMK, Humig, MIG, SCYB9, crg-10
Cible Humaine
Heure du test 1 heures 20 min
Durée totale du dosage 4 heures 45 min
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