Applied Biosystems™ MicroSEQ™ ID Microbial Identification Software, version 3.1.3

MicroSEQ ID Microbial Identification Software, version 3.1.3, allows you to easily identify and classify bacterial or fungal sequences by comparing them to a validated microbial library (available separately).

Features of MicroSEQ ID Microbial Identification Software include:

• Automatically identify unknown specimens against a validated microbial library of over 3000 microbial sequences
• Run plates directly on Applied Biosystems 3500 Series genetic analyzers
• Equipped with security, audit, and e-signature features to enable 21 CFR Pt. 11 compliance

Confidently identify microbes

The MicroSEQ ID sequence libraries (available separately) consist of rigorously validated bacterial and fungal libraries to ensure accurate identification. The MicroSEQ ID 16S rDNA 500 Microbial Library consists of over 2300 500-bp 16S rDNA sequences, including extensive coverage of gram-negative non-fermenters, Bacillus, Coryneforms, Mycobacteria, and Staphylococcus. The entries contain the first 500 bases of the 16S rRNA gene, sufficient to identify bacterial unknowns.

The MicroSEQ ID Fungal Gene Library contains 1300 reference sequences from the D2 region of the Large Subunit (rDNA) of yeast and mold species against which an unknown yeast or mold sequence can be compared in order to identify the organism. The MicroSEQ libraries contain information on correctly identified organisms and quality-controlled sequences derived from type strains of curated culture collections. The libraries also support sequence polymorphisms and include bacterial Category A biothreat agents.

Automatically identify unknown specimens

Reviewing each specimen in a project file to make a manual identification call can be tedious and time consuming. The Auto-ID feature in MicroSEQ ID software uses a set of editable parameters to automatically identify unknown specimens. The Results table is displayed in the report with an editable Comments field for additional information or manual identification entries.

Run plates directly

Applied Biosystems 3500 Series genetic analyzer users are able to run plates directly via the MicroSEQ ID software user interface. A single software wizard enables you to set up projects, specimens, and plates; adjust the plate layout via drag and drop; start and monitor the run; and review results.

The MicroSEQ system: a complete, integrated solution

Streamlining every microbial identification step, the MicroSEQ system combines the advantages of MicroSEQ ID software with easy-to-use PrepMan Ultra sample preparation reagents and protocols, MicroSEQ bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To help ensure optimal performance and system-wide integration, we develop and test all components of the system together. MicroSEQ ID software also includes security, audit, and electronic signature features, enabling the software to smoothly integrate into your workflow.