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MHC Class II (I-A/I-E) Rat anti-Mouse, Super Bright 645, Clone: M5/114.15.2, eBioscience™

Rat Monoclonal Antibody

Marke:  Invitrogen 64-5321-80

Additional Details : Gewicht : 0.01000kg

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Artikelnummer. 15825693

  • 196.00 CHF / 25 Mikrogramm


Verfügbar ab
26-05-2021
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Beschreibung

Beschreibung

Description: This DS5MMER monoclonal antibody recognizes mouse MerTK, a 170-210 kDa member of the TAM family of tyrosine kinase receptors that also includes Axl and Tyro3. MerTK is expressed on tissue macrophages and is involved in the removal of apoptotic cells. This process relies on two soluble ligands of MerTK, Protein S and Gas6 that bind to phosphatidylserine found on the outer leaflet of the plasma membrane of cells undergoing apoptosis. Upon binding these ligands, MerTK undergoes autophosphorylation at multiple tyrosine residues that activate the PI3K and Akt pathways. This results in the phagocytosis of apoptotic cells and also results in the direct inhibition of TLR-induced production of pro-inflammatory cytokines. In addition, MerTK may function as a putative entry receptor for filoviruses. Deficiency of MerTK causes general autoimmunity, inflammation and accumulation of apoptotic bodies. MerTK is constitutively released from the cell surface by metalloproteinases and thus is present in the serum and culture medium. This process can be enhanced by stimulation with LPS. MerTK can be used to help discriminate macrophages from dendritic cells. MerTK is often expressed on maligt cells and may be implicated in immune evasion. Applications Reported: This DS5MMER antibody has been reported for use in flow cytometric analysis. Applications Tested: This DS5MMER antibody has been tested by flow cytometric analysis of resident peritoneal macrophages.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product No. SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
Spezifikation

Spezifikation

MHC Class II (I-A/I-E)
Monoclonal
0.2 mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
P04231, P06342
H2-AB1, H2-EB1
IgG2b, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
14961, 14969
Flow Cytometry
M5/114.15.2
Super Bright 645
H2-Ab1
Liquid
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

For Research Use Only