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MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), eBioscience™, Invitrogen™
Rat Monoclonal Antibody
99.00 CHF - 343.00 CHF
Spezifikation
Antigen | MHC Class II (I-A/I-E) |
---|---|
Klon | M5/114.15.2 |
Konzentration | 0.5 mg/mL |
Anwendungen | Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Neutralization, Western Blot |
Klassifikation | Monoclonal |
Produktcode | Marke | Menge | Preis | Menge & Verfügbarkeit | |||||
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Produktcode | Marke | Menge | Preis | Menge & Verfügbarkeit | |||||
15217417
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Affymetrix eBioscience
14-5321-81 |
50 μg |
99.00 CHF
50 Mikrogramm |
Verfügbar ab: 02-05-2024
Loggen Sie sich ein, um den Lagerbestand zu sehen |
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15227417
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Affymetrix eBioscience
14-5321-82 |
100 μg |
154.00 CHF
100 Mikrogramm |
Lager vorrätigLoggen Sie sich ein, um den Lagerbestand zu sehen
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15237417
|
Affymetrix eBioscience
14-5321-85 |
500 μg |
343.00 CHF
500 Mikrogramm |
Lager vorrätigLoggen Sie sich ein, um den Lagerbestand zu sehen
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Beschreibung
Description: The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determit present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes. Applications Reported: This M5/114.15.2 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), immunohistology staining of frozen tissue sections, immunohistology staining of paraffin embedded tissue sections and in vitro blocking of T cell proliferative responses. (Please use Functional Grade purified M5/114.15.2, cat.16-4321, in functional assays.). Applications Tested: The M5/114.15.2 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.Spezifikation
MHC Class II (I-A/I-E) | |
0.5 mg/mL | |
Monoclonal | |
Liquid | |
RUO | |
PBS with 0.09% sodium azide; pH 7.2 | |
Abeta; AI323765; AI845868; cell surface glycoprotein; E-alpha-f; H-2 class II histocompatibility antigen, A beta chain; H-2 class II histocompatibility antigen, E-D alpha chain; H-2 class II histocompatibility antigen, E-K alpha chain; H-2 class II histocompatibility antigen, E-U alpha chain; H-2 class II histocompatibility antigen, I-E beta chain; H-2Ab; H2-Ab; H2-Ab1; H-2Ea; H2-Ea; H2-Ea-ps; H2Eb; H-2Eb; H2-Eb1; H2-iabeta; H2-IE-alpha; histocompatibility 2, class II antigen A, beta 1; histocompatibility 2, class II antigen E alpha; histocompatibility 2, class II antigen E alpha, pseudogene; histocompatibility 2, class II antigen E beta; Ia2; Ia-2; Ia3; Ia-3; Ia4; Ia-4; IAb; I-Ab; I-Abeta; I-Ad; I-Aq; I-E alpha MHC class II; I-E beta MHC class II; I-Ealpha; I-Ed; I-Ek; major histocompatibility complex class II beta chain; major histocompatibility complex H-2E beta chain; MHC class 2; MHC class II antigen A beta; MHC class II antigen E alpha; MHC class II H2-IA-beta-psi; MHC class II I | |
H2-Ab1, H2-Eb1 | |
Primary | |
4° C | |
H2-Ab1 |
M5/114.15.2 | |
Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Neutralization, Western Blot | |
Unconjugated | |
Rat | |
Mouse | |
P01921, P04230, P06342, P06343, P06344, P06345, P06346, P14483 | |
14961, 14969 | |
IgG2b κ | |
Affinity chromatography | |
Antibody |
For Research Use Only.