missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Biochemical Reagents
  4. Transcription and Translation Reagents
  5. Invitrogen™ MegaScript™ T7 Transcription Kit Plus

Invitrogen™ MegaScript™ T7 Transcription Kit Plus

Codice prodotto. 31662351
Cambia vista
Click to view available options
N. di reazioni:
25 Reactions
50 Reactions
Dimensione della confezione:
Pezzo
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Codice prodotto. N. di reazioni Dimensione della confezione
31662351 25 Reactions -
17945051 50 Reactions Pezzo
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
Les retours ne sont pas autorisés pour ce produit. Consulta la politica di reso
Codice prodotto. 31662351 Fornitore Invitrogen™ N. del fornitore A5762225
 Articolo soggetto a restrizioni
Lager vorrätig
Aggiungi al carrello
Aggiungi al carrello

missing translation for 'validMainframeMsg'

Les retours ne sont pas autorisés pour ce produit. Consulta la politica di reso

Descrizione

The MegaScript T7 Transcription Kit Plus contains all the reagents and buffers necessary for in vitro synthesis of large amounts of RNA, is suitable for full- or partially-modified nucleotide substitution to produce modified RNA, and enables the use of different cap analogs.

The MegaScript T7 Transcription Kit Plus contains all the reagents and buffers necessary for in vitro synthesis of large amounts of RNA. Each 20-μL reaction yields an average of 100 μg of RNA, while the scaled-up protocol yields up to 1 mg of RNA. This kit is suitable for full- or partially-modified-nucleotide substitution to produce modified RNA. It is also suitable for co-transcriptional capping with cap analogs to make capped RNA that can be translated into protein. Easily scale from RUO to GMP with the incorporation of TheraPure reagents, eliminating reoptimization during scale up.

Features of the MegaScript T7 Transcription Kit Plus include:

  • Optimized to achieve high mRNA yields (>5 mg/mL)
  • Flexible—allows use of modified nucleotides and/or cap analogs such as ARCA or CleanCap
  • Includes GFP control template
  • Made up of high quality reagents (TheraPure); eliminates the need to qualify new reagents during scale-up
  • Available in two sizes: 50 and 1000 reactions

The MegaScript T7 Transcription Kit Plus requires a linearized DNA template with a T7 RNA polymerase promoter to create >5 mg/mL of RNA. MEGAscript kits are intended for the synthesis of large amounts of unlabeled or low-specific-activity RNA for a variety of uses, including transfections, preclinical mRNA therapeutic mRNA studies, microinjections, in vitro translation, and RNA structure and function analysis. Note that this kit does not create capped RNA, which is necessary for translational studies. MEGAscript kits are not recommended for synthesis of high-specific-activity probes.

TRUSTED_SUSTAINABILITY

Specifica

Tipo di cellula HEK293, 293, HeLa, CHO-K1, CHO, Jurkat, Primary Human T Cells, Primary Cells, Stem Cells, iPSC, NIH-3T3, HepG2, A549, HUVEC
Final Product Type Synthetic mRNA
Formato Animal Origin Free Kit
Isolation Technology Silica Spin Column
Purification Target mRNA
Tipo di campione mRNA, Total RNA
N. di reazioni 25 Reactions
Linea di prodotti MEGAscript
Product Type T7 In Vitro Transcription Kit
Quantità 25 Reactions
Promoter T7
Shipping Condition Dry Ice
Resa >100 μg
Starting Material PCR Products, Linearized DNA Plasmid
Vedi altri risultati Mostra meno risultati

Esclusivamente per scopi di ricerca.

Titolo del prodotto
Selezionare un problema

Facendo clic su Invia, l'utente riconosce che potrebbe essere contattato da Fisher Scientific in merito al feedback fornito in questo modulo. Non condivideremo le vostre informazioni per altri scopi. Tutte le informazioni di contatto fornite saranno conservate in conformità con la nostra Politica sulla privacy. Informativa sulla privacy.